Paper Information

Journal:   AGRICULTURAL SCIENCES AND TECHNOLOGY   2006 , Volume 20 , Number 7; Page(s) 163 To 171.
 
Paper: 

INVESTIGATION OF LEAF PROTEOME VARIATION IN RESPONSE TO THE ASCOCHYTA BLIGHT DISEASES INFECTION IN SENSITIVE AND RESISTANT GENOTYPES OF CHICKPEA

 
 
Author(s):  GIVECHI H., BAGHERI A., SHOKOUHIFAR F.
 
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Abstract: 
Fungal diseases are the most important biotic stresses in plant production.
Identification of proteins that are synthesized in response to the disease and also identification of the coding genes of these proteins is an important task. In this study, proteome variation in response to the ascochyta blight diseases was investigated before and after disease indvetion in sensitive and resistant genotypes of chickpea.
Protein profiles of the lines were obtained using SDS-PAGE electrophoresis.
Difference was observed in proteins with molecular weight 8.6, 19, 24 and 26 kDa. The expression of the first protein decreased and the other proteins increased in comparison with control. In resistant geno; ypes, higher expression was observed in 19, 24 and 26, kDa proteins in comparison with sensitive genotypes.
Higher expression of these proteins has been observed in resistant genotypes especially in high resistant genotype MCC496. Non expression or low expression is observed in sensitive genotypes especially MCC506. According to the results, it seems that expression of the identified proteins is related to pathogen attacks. Also the higher expression level of these proteins in resistant genotypes in comparison with susceptible genotypes could indicate the role of these proteins to establish resistance.
 
Keyword(s): SENSITIVE AND RESISTANT GENOTYPES, ASCOCHYTA BLIGHT, DEFENSE PROTEINS, SDS-PAGE
 
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