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Paper Information

Journal:   AGRICULTURAL SCIENCES AND TECHNOLOGY   2006 , Volume 20 , Number 7; Page(s) 137 To 145.
 
Paper: 

PRODUCTION OF TRANSGENIC HYOSCYAMUS MUTICUS VIA TRANSFORMATION BY AGROBACTERIUM RHIZOGENES

 
 
Author(s):  MAHMOODNIA MEIMAND M., FARSI M., MARASHI H., SHAHRIARI F., ZOLALA J.
 
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Abstract: 
Hyoscyamus plants are important sources of tropane alkaloids which are produced in roots and transferred to aerial parts of the plants. Agrobacterium rhizogenes causes hairy root disease in host plants such as solanaceae. Hairy roots which are induced on inoculated explants by A. rhizogenes, grow faster than normal roots and they are genetically stable.
Furtheremore they can regenerate whole transgenic plants.
Therefore, there is a possibility that transgenic H. muticus plants produce more alkaloid compared with normal ones. This research was conducted with the aim of studying genetic stability in transgenic plants and their progenies and compairing morphological traits in transgenic plants with normal counterparts. Leaf and nodal segments of Hyoscyamus plants were inoculated by A. rhizogenes strain LBA9402. When hairy roots symptoms appeared, liquid cultures were established in B5 medium without growth regulators. For plant regeneration, calli derived from hairy roots were transferred to free hormone MS and B5 medium and also to MS medium contaning different levels of auxins and cytokinins. Considerable differences in morphological traits were observed among regenerated plants as well as between transgenic plants with control ones.
Transgenic plants were considerably diverse in leaf size, leaf shape, flower shape and seed capability to produce flower and seed. Transgenic nature of regenerated plants and their progenies were confirmed through PCR by amplifying a specific fragment for roLAB genes of bacterial T-DNA.
 
Keyword(s): HYOSCYAMUS MUTICUS, AGROBACTERIUM RHIZOGENES, HAIRY ROOTS, TROPANE ALKALOIDS
 
References: 
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