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Paper Information

Journal:   AGRICULTURAL SCIENCES AND TECHNOLOGY   2006 , Volume 20 , Number 7; Page(s) 61 To 69.
 
Paper: 

STUDY OF GENETIC DIVERSITY OF PERSIAN CLOVER POPULATIONS USING RAPD MARKER

 
 
Author(s):  SAMEI K., ARZANI A., MIRMOHAMMADI MAIBODY A.M.
 
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Abstract: 

Field crop landraces are valuable sources of genetic variations. Knowledge and implication of these variations are critical in the plant breeding programs.
Legume forage clover due to high forage yield, quality, nitrogen fixation and improvement of soil textures is cultivated worldwide. Persian clover (Trifolium resupinatum L.), with Iranian origin, is one of the prevalent clover species grown worldwide. Molecular markers are used efficiently for assessment of genetic diversity in crop plants.
In this study 20 Persian clover populations collected from different areas were used. DNA extractions were carried out using mini preparation method with equal amount of leaves from 30 plants of each population. DNA samples from 20 populations of clover were evaluated using RAPD markers.
Eight primers out of 30 used primers produced repeatable bands.
Cluster analysis was conducted using NTSYS software and UPGMA method based on Jaccard's similarity matrix. Primers totally produce 75 bands, of which 66 bands (88%) were polymorphic among clover genotypes. The greatest and least amplification fragments belonged to OPHl2 and OPG06 primers, respectively and average band number of primers was estimated as 9.37 bands. According to cluster analysis and cutting dendrogram in 0.7 similarity coefficients, clover populations divided into seven groups in which Kazerun, Kermanshahi (2) and Haftun Isfahan individually formed a separate clusters. According to similarity matrix, the least similarity (36%) belonged to Haftchin Brujerd and Kazerun and the highest similarity belonged to Chegeni and Haftchin Hamedan. Clustering based on RAPD method almost substantiated the grouping based on geographical origin. Considering the results, it is concluded that RAPD technique can be used for genetic diversity study of Persian clover as well as discrimination of it's cultivars.

 
Keyword(s): PERSIAN CLOVER, TRIFOLIUM RESUPINATUM L., GENETIC DIVERSITY, RAPD MARKER
 
References: 
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