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Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCES   JANUARY 2008 , Volume 13 , Number 2 (SUPPLEMENT); Page(s) 341 To 355.
 
Paper: 

COMPARATIVE STUDY ON ENZYMATIC CHANGES IN TRICHOGRAMMA BRASSICAE BEZD., LABORATORY AND CORN-RICE FIELD POPULATIONES OF MAZANDARAN REGION

 
 
Author(s):  MOAREFI M., EBRAHIMI E., SHOJAEI M., OSTOVAN H.
 
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Abstract: 

In modem biosystematics, biochemichal and morphometric basis are fundamentals of evolutionary taxonomy which its biotechnology shows the intrapopulation evolution in different environmental and mass-rearing situations in comparison with natural host population in agroecosystems. Our research program was based on enzymatic study and in comparison with morphometric results identification of Trichogramma brassicae Bezd, Parasitoids of rice stemborer (Chilo suppressalis WLK.) and com stemborer (Ostrinia nubilalis var: persica HBN,) in different fields of Mazandaran, also in comparison with different population of Trichogramma brassicae feeding on mass-rearing host, Ephestia kuehniella Zell Morphological characters of T. brassicae were studied by means of male genitalia and antennae. Electrophoretic technique have been used for enzymatic study. Polyacrylarnid gels molded between glass plates. For enzyme polymorphism included 12 populations, 20 adults descendants of virgin females in which esterases (EST) were analyzed. The genetic identity distances were calculated based on Nei (1972) method. Based on dendogram, difference between natural and mass-reared populations were observed. Results from enzymatic and morphometric investigations indicated that there is significant differences (95%-99%) between these populations. The efficacy of biological control is dependent on the quality of the T. brassicae. Quality control has always been an important factor in biocontrol. The selection of suitable and adaptive species, quality of mass-reared and climate conditions at the time of release are the main factors that determine the efficacy of using Trichogramma brassicae in Mazandaran. In the other hand for utmost effect, we have to improve the quality control.

 
Keyword(s): TRICHOGRAMMA BRASSICAE, ELECTROPHORESIS, ESTERASE ENZYME, SYSTEMATIC
 
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