Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY   JULY-SEPTEMBER 2012 , Volume 6 , Number 2; Page(s) 111 To 116.
 
Paper: 

Nitric Oxide-Induced Polycystic Ovaries In The Wistar Rat

 
 
Author(s):  Hasani Fatemeh, Karami Manizheh*, Jalali Nadoushan Mohammadreza, Eftekhari Yazdi Poopak
 
* DEPARTMENT OF BIOLOGY, FACULTY OF BASIC SCIENCES, SHAHED UNIVERSITY, TEHRAN, IRAN
 
Abstract: 
Background: Nitric oxide (NO) involves in polycystic ovary syndrome (PCOS), a cause of infertility in women during the reproductive age. The PCOS is now categorized as an inflammatory phenomenon. The aim of this study was to evaluate the role of NO, a proinflammatory agent, in this syndrome at histological and biochemical levels.Materials and Methods: In this experimental study, animals were female Wistar rats (weighing 200-250 g) kept under standard conditions. L-Arginine (50-200 mg/kg), a precursor of NO, was injected intra-peritoneally (i.p.) through a period ranging from 9 to14 days/ once a day. The rats' estrous cycle was studied using Papanicolaou test; those showing phase of Diestrous were grouped into experimental and control groups. The control group solely received saline (1 ml/kg, i.p.) throughout all experiments. To evaluate the inflammatory effect of NO, the rats were treated an anti-inflammatory agent, naloxone hydrochloride (0.4 mg/kg, i.p.), prior to L-arginine. At the end of the treatment period all animals’ ovaries were assessed for histopathological and histochemical investigations. Also, activation of NO synthase (NOS) in the experiments was studied using NADPH-diaphorase technique.Results: The ovaries of rats treated with L-arginine showed polycystic characteristics in contrast to those collected from control or naloxone pretreated groups, based on image analysis. A difference in enzyme activation was also shown in the sections that belonged to the groups that received L-arginine when compared with the pre-naloxone and control groups.Conclusion: Based on these results, we believe that NO may play a major role in the pathophysiology of PCOS.
 
Keyword(s): PCOS, NITRIC OXIDE, L-ARGININE, NALOXONE, NADPH-DIAPHORASE
 
 
International related papers: 
 
Most related Highly related Moderately related Least related
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
 
+ Click to Cite.
APA: Copy

HASANI, F., & KARAMI, M., & JALALI NADOUSHAN, M., & EFTEKHARI YAZDI, P. (2012). NITRIC OXIDE-INDUCED POLYCYSTIC OVARIES IN THE WISTAR RAT. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 6(2), 111-116. https://www.sid.ir/en/journal/ViewPaper.aspx?id=273399



Vancouver: Copy

HASANI FATEMEH, KARAMI MANIZHEH, JALALI NADOUSHAN MOHAMMADREZA, EFTEKHARI YAZDI POOPAK. NITRIC OXIDE-INDUCED POLYCYSTIC OVARIES IN THE WISTAR RAT. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY. 2012 [cited 2022January26];6(2):111-116. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=273399



IEEE: Copy

HASANI, F., KARAMI, M., JALALI NADOUSHAN, M., EFTEKHARI YAZDI, P., 2012. NITRIC OXIDE-INDUCED POLYCYSTIC OVARIES IN THE WISTAR RAT. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, [online] 6(2), pp.111-116. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=273399.



 
  pdf-File
Yearly Visit 60
 
 
Latest on Blog
Enter SID Blog