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Paper Information

Journal:   INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY   SUMMER 2011 , Volume 5 , Number SUPPLEMENT 1; Page(s) 77 To 78.
 
Paper: 

GENETICS: RETINOIC ACID AND STO CO-CULTURE SYSTEM ENHANCE MOUSE EMBRYONIC STEM CELL–DERIVED GERM CELL PROLIFERATION

 
 
Author(s):  MAKOOLATI Z.*, MOVAHEDIN M., FOROUZANDEH MOGHADAM M.
 
* DEPARTMENT OF ANATOMICAL SCIENCE, MEDICAL SCIENCES FACULTY, FASA UNIVERSITY, FASA, IRAN
 
Abstract: 

Background: An in vitro system that supports primordial germ cells (PGCs) survival and proliferation is useful for enhancement of these cells and efficient transplantation. One approach is cultivation of PGCs under proper conditions that allow self-renewal and proliferation of PGCs. For this purpose, we compared the effects of different concentrations of retinoic acid (RA), and the effect of PGCs co-culture with STO cells on the proliferation of ESCs- derived PGCs.
Materials and Methods: CCE mouse ESCs were cultured in DMED containing 20% fetal bovine serum (FBS) for 1 day in order to embryoid body (EB) formation and then cultured 4 days in simple culture medium in the presence of 5 ng/ml BMP4. ESCs derived germ cells cultured for 7 days in the presence of different doses (0-5 µM) of RA both in the simple and STO co-culture systems. Expression of a6 integrin, the germ cell related gene, was evaluated using quantitative PCR. Data analyses were done with ANOVA and Tukey post test.
Results: The comparison of gene expression between different groups showed that α6 integrin was expressed in a significant higher rate in 2 µM RA concentration in the STO co-culture system (p0.05).
Conclusion: Quantitative RT-PCR was used to estimate the level of germ cell gene expression. The results confirmed that the addition of 2 µM RA concentrations on the top of the STO feeder layer improve the proliferation of ESCs-derived PGCs.

 
Keyword(s): EMBRYONIC STEM CELL, RA, GERM CELL, α6 INTEGRIN
 
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