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Paper Information

Journal:   THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)   FALL 2012 , Volume 9 , Number 3 (36); Page(s) 214 To 225.
 
Paper: 

CLONING AND TRANSIENT EXPRESSION OF CYTOPROTECTIVE FACTOR, HO-1, IN MESENCHYMAL STEM CELLS USING THE ADENOVIRAL EXPRESSION SYSTEM THROUGH GATEWAY TECHNOLOGY

 
 
Author(s):  HAMEDI ASL P., HALABIAN R., MOHAMADZADEH M., MOHAMMADIPOUR M., BAKHSHANDEH Z., HAMEDI ASL D., KIANI A.A., JALILI M., AMIRIZADEH N., HABIBI ROUDKENAR M.*
 
* BLOOD TRANSFUSION RESEARCH CENTER, HIGH INSTITUTE FOR RESEARCH AND EDUCATION IN TRANSFUSION MEDICINE, TEHRAN, IRAN
 
Abstract: 

Background and Objectives: Heme oxegenase1 (HO-1) is one of the potent cytoprotective factors. The goal of this study was to perform cloning and transient over expression of the human HO-1 gene in mesenchymal stem cells (MSCs) using the adenoviral expression system based on the gateway technology.
Materials and Methods: In order to induce expression of HO-1, A549 cell lines were exposed to UV for 1 hour. The full length cDNA of HO-1 was isolated and cloned into pENTR TOPO/D vector by TOPO cloning reaction. To construct the expression clone, a LR recombination reaction was carried out between the entry clone and destination vector, pAd/CMV/V5-DEST. The recombinant virus was produced in the appropriate mammalian cell line. MSCs were infected by the recombinant virus expressing HO-1.
Results: The results showed that human recombinant HO-1 was successfully cloned and the accuracy of the gene and its frame in the vector were confirmed by DNA sequencing. Expression of HO-1 in MSCs was confirmed by RT-PCR and western blot analysis. The results indicated that the expression of HO-1 is transient.
Conclusions: Transient expression of human HO-1 gene in MSCs by using adenovirus expression system may be considered as an efficient gene transfer strategy into MSCs in order to promote stem cell therapy.

 
Keyword(s): MESENCHYMAL STEM CELLS, HEME OXYGENASE-1, GENETIC ENGINEERING, ADENOVIRUSES
 
 
References: 
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Click to Cite.
APA: Copy

HAMEDI ASL, P., & HALABIAN, R., & MOHAMADZADEH, M., & MOHAMMADIPOUR, M., & BAKHSHANDEH, Z., & HAMEDI ASL, D., & KIANI, A., & JALILI, M., & AMIRIZADEH, N., & HABIBI ROUDKENAR, M. (2012). CLONING AND TRANSIENT EXPRESSION OF CYTOPROTECTIVE FACTOR, HO-1, IN MESENCHYMAL STEM CELLS USING THE ADENOVIRAL EXPRESSION SYSTEM THROUGH GATEWAY TECHNOLOGY. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 9(3 (36)), 214-225. https://www.sid.ir/en/journal/ViewPaper.aspx?id=272906



Vancouver: Copy

HAMEDI ASL P., HALABIAN R., MOHAMADZADEH M., MOHAMMADIPOUR M., BAKHSHANDEH Z., HAMEDI ASL D., KIANI A.A., JALILI M., AMIRIZADEH N., HABIBI ROUDKENAR M.. CLONING AND TRANSIENT EXPRESSION OF CYTOPROTECTIVE FACTOR, HO-1, IN MESENCHYMAL STEM CELLS USING THE ADENOVIRAL EXPRESSION SYSTEM THROUGH GATEWAY TECHNOLOGY. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON). 2012 [cited 2021May06];9(3 (36)):214-225. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272906



IEEE: Copy

HAMEDI ASL, P., HALABIAN, R., MOHAMADZADEH, M., MOHAMMADIPOUR, M., BAKHSHANDEH, Z., HAMEDI ASL, D., KIANI, A., JALILI, M., AMIRIZADEH, N., HABIBI ROUDKENAR, M., 2012. CLONING AND TRANSIENT EXPRESSION OF CYTOPROTECTIVE FACTOR, HO-1, IN MESENCHYMAL STEM CELLS USING THE ADENOVIRAL EXPRESSION SYSTEM THROUGH GATEWAY TECHNOLOGY. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), [online] 9(3 (36)), pp.214-225. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272906.



 
 
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