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Paper Information

Journal:   THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)   FALL 2012 , Volume 9 , Number 3 (36); Page(s) 199 To 213.
 
Paper: 

EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34+ CELLS

 
 
Author(s):  HASHEMI Z.S., FOROUZANDEH MOGHADAM M.*, SOLEIMANI M., KHAMISIPOUR GH., MOSSAHEBI MOHAMMADI M.
 
* TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background and Objectives: Bone marrow transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming by minimum manipulation, mineralized bone allograft (MBA) scaffold coated by UCB-USSC (unrestricted somatic stem cells) was used to expand CD34+ cells from UCB.
Materials and Methods: In this experimental study, UCB-USSCs were isolated and characterized by morphologic and immunophenotypical analysis. Then they were seeded on MBA (for 3D figuration) and culture plate (for 2D figuration) as a feeder layer. CD34+ cells were isolated from the UCB (by MACS method) and were expanded in 2D and 3D conditions for 3 weeks. At the end, cell count, flow cytometry, cologenic assay, and LTC-IC were done.
Results: After 3 weeks, ex vivo expansion of UCB-CD34+ was enhanced 250±13.2 in 3D. The highest CFC expansion and LTC-IC were observed at day 14. Flow cytometry analysis showed the lowest percentage in 3D culture.
Conclusions: USSCs by 3D MBA structure produce the hematopoietic cytokine, extracellular matrix and binding molecules, they also create the intercellular interaction that can be used as a suitable feeder layer. It provides anex vivo mimicry of bone marrow niche by enhancing surface/volume ratio. This model could be a suitable and alternative environment for HSCs expansion and hematopoiesis.

 
Keyword(s): MBA, HEMATOPOIETIC STEM CELLS, UMBILICAL CORD BLOOD, ALLOGRAFT
 
 
References: 
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Citations: 
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APA: Copy

HASHEMI, Z., & FOROUZANDEH MOGHADAM, M., & SOLEIMANI, M., & KHAMISIPOUR, G., & MOSSAHEBI MOHAMMADI, M. (2012). EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34+ CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 9(3 (36)), 199-213. https://www.sid.ir/en/journal/ViewPaper.aspx?id=272897



Vancouver: Copy

HASHEMI Z.S., FOROUZANDEH MOGHADAM M., SOLEIMANI M., KHAMISIPOUR GH., MOSSAHEBI MOHAMMADI M.. EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34+ CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON). 2012 [cited 2021June20];9(3 (36)):199-213. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272897



IEEE: Copy

HASHEMI, Z., FOROUZANDEH MOGHADAM, M., SOLEIMANI, M., KHAMISIPOUR, G., MOSSAHEBI MOHAMMADI, M., 2012. EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34+ CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), [online] 9(3 (36)), pp.199-213. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272897.



 
 
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