Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)   FALL 2012 , Volume 9 , Number 3 (36); Page(s) 199 To 213.
 
Paper: 

EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34+ CELLS

 
 
Author(s):  HASHEMI Z.S., FOROUZANDEH MOGHADAM M.*, SOLEIMANI M., KHAMISIPOUR GH., MOSSAHEBI MOHAMMADI M.
 
* TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background and Objectives: Bone marrow transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming by minimum manipulation, mineralized bone allograft (MBA) scaffold coated by UCB-USSC (unrestricted somatic stem cells) was used to expand CD34+ cells from UCB.
Materials and Methods: In this experimental study, UCB-USSCs were isolated and characterized by morphologic and immunophenotypical analysis. Then they were seeded on MBA (for 3D figuration) and culture plate (for 2D figuration) as a feeder layer. CD34+ cells were isolated from the UCB (by MACS method) and were expanded in 2D and 3D conditions for 3 weeks. At the end, cell count, flow cytometry, cologenic assay, and LTC-IC were done.
Results: After 3 weeks, ex vivo expansion of UCB-CD34+ was enhanced 250±13.2 in 3D. The highest CFC expansion and LTC-IC were observed at day 14. Flow cytometry analysis showed the lowest percentage in 3D culture.
Conclusions: USSCs by 3D MBA structure produce the hematopoietic cytokine, extracellular matrix and binding molecules, they also create the intercellular interaction that can be used as a suitable feeder layer. It provides anex vivo mimicry of bone marrow niche by enhancing surface/volume ratio. This model could be a suitable and alternative environment for HSCs expansion and hematopoiesis.

 
Keyword(s): MBA, HEMATOPOIETIC STEM CELLS, UMBILICAL CORD BLOOD, ALLOGRAFT
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 73
 
Latest on Blog
Enter SID Blog