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Paper Information

Journal:   INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY   SUMMER 2011 , Volume 5 , Number SUPPLEMENT 1; Page(s) 43 To 44.
 
Paper: 

ANDROLOGY: DHR123: A SUITABLE FLUORESCENT MITOCHONDRIAL PROBE FOR ASSESSMENT OF H2O2 IN HUMAN SPERMATOZOA

 
 
Author(s):  KIANI ESFAHANI A.*, TAVALAEE M., DEEMEH M.R., HAMIDITABAR M.A., NASR ESFAHANI M.H.
 
* DEPARTMENT OF REPRODUCTION AND DEVELOPMENT, REPRODUCTIVE BIOMEDICINE CENTER, ROYAN INSTITUTE FOR ANIMAL BIOTECHNOLOGY, A.C.E.C.R., ISFAHAN, IRAN
 
Abstract: 

Background: The objective of present study was to assess potential of DHR123 (dihydrorhodamine 123) fluorescent probe for measurement of H2O2 produced by human spermatozoa and comparing the results with DCFH-DA (2’, 7’-dichlorodihydrofluorescein diacetate).
Materials and Methods: Fluorescent intensity and percentage R123 and DCF positive sperm measured by flow cytometry. The suitable condition for assessment of H2O2 produced by sperm was 0.05
mM for 1 million sperm per ml for 20 minutes.
Results: The results of H2O2 measurement showed a significant correlation (r=0.818, p<0.001) with the DCFH-DA staining routinely used for assessment of H2O2 produced by spermatozoa. H2O2 production was detectable by DHR123 staining in sperm mid-piece.
Conclusion: DHR123 is a suitable fluorescent probe for assessment of H2O2 produced by human spermatozoa and can present heterogeneity in H2O2 production between different samples.

 
Keyword(s): SPERM, H2O2, DHR123, R123, DCFH-DA, FLOW CYTOMETRY
 
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