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Paper Information

Journal:   INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY   SUMMER 2011 , Volume 5 , Number SUPPLEMENT 1; Page(s) 43 To 43.
 
Paper: 

ANDROLOGY: A NEW MORPHOLOGY STAINING METHOD FOR SPERM OF SOME DOMESTIC ANIMALS

 
 
Author(s):  JANAT ALIPOUR F.*, FAKHERI R., SHAHVERDI A.H., DANESHZADEH M.T., GOLMOHAMMADI P.
 
* DEPARTMENT OF EMBRYOLOGY, REPRODUCTIVE BIOMEDICINE CENTER, ROYAN INSTITUTE FOR REPRODUCTIVE BIOMEDICINE, A.C.E.C.R., TEHRAN, IRAN
 
Abstract: 

Background: There are some methods for morphology staining of sperm. Although many different and also new methods are used in semen analysis, light microscopy is still used for routine morphological evaluation. The most used of the methods is Papanicolaou staining. This method is routinely used in Andrology laboratory for Human sperm morphology staining. In some domestic animals such as bull, Ram and Goat this method is not efficient for sperm staining.
Materials and Methods: In this study Papanicolaou method was altered by deleting Hematoxylin dye and adding Giemsa dye to better showing the parameters of sperm, especially acrosome in sperm of domestic animal.
Results: By comparison stained slides under the light microscopy results of the study showed that in some animals such as Goat, Ram and Bull sperm is better stained by this method.
Conclusion: According to inpermability of some domestic animals sperm to Papanicolaou dyes and light microscopy comparison we conclued that this method with regards to be fast and inexpensive can use for staining in some animals sperm.

 
Keyword(s): SPERM MORPHOLOGY, GIEMSA STAINING METHOD, DOMESTIC ANIMALS
 
 
References: 
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Click to Cite.
APA: Copy

JANAT ALIPOUR, F., & FAKHERI, R., & SHAHVERDI, A., & DANESHZADEH, M., & GOLMOHAMMADI, P. (2011). ANDROLOGY: A NEW MORPHOLOGY STAINING METHOD FOR SPERM OF SOME DOMESTIC ANIMALS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 5(SUPPLEMENT 1), 43-43. https://www.sid.ir/en/journal/ViewPaper.aspx?id=272408



Vancouver: Copy

JANAT ALIPOUR F., FAKHERI R., SHAHVERDI A.H., DANESHZADEH M.T., GOLMOHAMMADI P.. ANDROLOGY: A NEW MORPHOLOGY STAINING METHOD FOR SPERM OF SOME DOMESTIC ANIMALS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY. 2011 [cited 2021May13];5(SUPPLEMENT 1):43-43. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272408



IEEE: Copy

JANAT ALIPOUR, F., FAKHERI, R., SHAHVERDI, A., DANESHZADEH, M., GOLMOHAMMADI, P., 2011. ANDROLOGY: A NEW MORPHOLOGY STAINING METHOD FOR SPERM OF SOME DOMESTIC ANIMALS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, [online] 5(SUPPLEMENT 1), pp.43-43. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272408.



 
 
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