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Paper Information

Journal:   INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY   SUMMER 2012 , Volume 6 , Number SUPPLEMENT 1; Page(s) 136 To 137.
 
Paper: 

CHRONIC ALCOHOLISM INDUCED MALE INFERTILITY: IMPAIRMENT OF SPERM QUALITY

 
 
Author(s):  SHOJAZADEH SH.*, AHMADI A., GHODRATI GHOLENJI S.
 
* LABORATORY OF EMBRYOLOGY, DEPARTMENT OF BASIC SCIENCE, FACULTY OF VETERINARY MEDICINE, URMIA UNIVERSITY, URMIA, IRAN
 
Abstract: 
Background: Prolonged alcohol abuse in men can cause testosterone deficiency and shrinkage of the testicles (testicular atrophy). This can lead to impotence, sterility and ‘feminisation’.
When alcohol is broken down in the body, it may also interfere with normal sperm structure and movement. This study was designed to determine the effects of administration of alcohol on sperm quality, sperm maturation and DNA damage Materials and Methods: Twenty adult male mice were divided in 2 groups: control sham (salin normal) and test group received ethanol (3 g/kg body weight as 25%, v/v) was given by gastric intubation daily for 45 days.
After 45 days all mice were sacrificed and cauda epididymis were removed and placed in 1ml HTF+BSA 4mg/ ml and incubated for 30 min in Co2 incubator (5% Co2, 37oC) to allow the spermatozoa to swim out (p<0.05).
The spermatozoal suspension was analyzed for sperm motility, concentration in the cauda epididymis, viability and sperm chromatin quality and DNA integrity was assessed by Aniline Blue and Acridine Orange staining following sperm sample.
Results: This study showed that treatment with ethanol caused significant decrease in sperm concentration in cauda epididymis, motility, and viability, while abnormal sperms increased as compared to control. These changes were associated with significant increase in DNA damage and chromatin abnormality in the cauda epididymal spermatozoa as evidenced by Acridine Orange (AO) and Aniline Blue staining respectively (p<0.05).
Conclusion: The present study demonstrates that chronic consumption of ethanol has toxic effect on spermatozoa and impairs fertility in male mice. Although it is hypothesized that chronic consumption of ethanol induced testicular injury with adverse effect on sperm production, sperm DNA integration and nuclear maturation.
 
Keyword(s): ALCOHOL, SPERM, VIABILITY, DNA DAMAGE, MOUSE
 
 
References: 
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Click to Cite.
APA: Copy

SHOJAZADEH, S., & AHMADI, A., & GHODRATI GHOLENJI, S. (2012). CHRONIC ALCOHOLISM INDUCED MALE INFERTILITY: IMPAIRMENT OF SPERM QUALITY. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 6(SUPPLEMENT 1), 136-137. https://www.sid.ir/en/journal/ViewPaper.aspx?id=272346



Vancouver: Copy

SHOJAZADEH SH., AHMADI A., GHODRATI GHOLENJI S.. CHRONIC ALCOHOLISM INDUCED MALE INFERTILITY: IMPAIRMENT OF SPERM QUALITY. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY. 2012 [cited 2021May11];6(SUPPLEMENT 1):136-137. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272346



IEEE: Copy

SHOJAZADEH, S., AHMADI, A., GHODRATI GHOLENJI, S., 2012. CHRONIC ALCOHOLISM INDUCED MALE INFERTILITY: IMPAIRMENT OF SPERM QUALITY. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, [online] 6(SUPPLEMENT 1), pp.136-137. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272346.



 
 
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