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Paper Information

Journal:   INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY   SUMMER 2012 , Volume 6 , Number SUPPLEMENT 1; Page(s) 127 To 128.
 
Paper: 

THE RATE OF IN VITRO OOCYTE MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT IN PRESENCE OF MELATONIN IN MICE

 
 
Author(s):  BAHADORI M.H.*, ASGARI Z., RAMEZANI M.
 
* CELLULAR AND MOLECULAR RESEARCH CENTER, MEDICINE FACULTY, GUILAN UNIVERSITY OF MEDICAL SCIENCES, RASHT, IRAN
 
Abstract: 

Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger.
Therefore, effect of melatonin during in vitro oocyte maturation (IVM), fertilization and embryo development of mouse oocytes was evaluated.
Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cumulus–oocyte complexes (COCs, group i) and denuded COC (d-COC, group ii). The oocytes were cultured in maturation medium with different dosages of melatonin (1- 110-105 nM). The cumulus expansion and nuclear status were evaluated after 24 hours of in vitro maturation. The oocytes were used for in vitro fertilization (IVF). The fertilized oocytes were cultured in medium supplemented with different doses of melatonin.
Results: The expansion (86.79%) and maturation (80.55%) rate of COCs increased in supplemented medium with 10 nM of melatonin Vs control group (73.33%, p<0.01), but oocytes without cumulus cells indicated higher maturation rate at higher melatonin doses (10 and 100
mM, 84.34 and 79.5% vs.69.33% in control group (p<0.01). Fertlization rate was higher in treated medium with 1 mM of melatonin (93.75 %, p<0.01). The rate of cleavage and blastocyst formation was promoted in medium supplemented with 10 and 100 nM of melatonin (92.37 and 89.36% vs.81.25% in control group (p<0.01).
We observed a dose dependent response to melatonin treatment in this experiment.
Conclusion: Exogenous melatonin can promote cumulus cell expansion, IVM, and embryo development.
However we investigated a dose-dependent response in different stages of maturation and development. It may reflect sensitive rate of oocytes and embryos to culture conditions.

 
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APA: Copy

BAHADORI, M., & ASGARI, Z., & RAMEZANI, M. (2012). THE RATE OF IN VITRO OOCYTE MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT IN PRESENCE OF MELATONIN IN MICE. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 6(SUPPLEMENT 1), 127-128. https://www.sid.ir/en/journal/ViewPaper.aspx?id=272181



Vancouver: Copy

BAHADORI M.H., ASGARI Z., RAMEZANI M.. THE RATE OF IN VITRO OOCYTE MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT IN PRESENCE OF MELATONIN IN MICE. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY. 2012 [cited 2021July30];6(SUPPLEMENT 1):127-128. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272181



IEEE: Copy

BAHADORI, M., ASGARI, Z., RAMEZANI, M., 2012. THE RATE OF IN VITRO OOCYTE MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT IN PRESENCE OF MELATONIN IN MICE. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, [online] 6(SUPPLEMENT 1), pp.127-128. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272181.



 
 
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