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Journal:
INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY
SUMMER 2011
, Volume 5
, Number SUPPLEMENT 1; Page(s) 25 To 25. |
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Paper: ANDROLOGY: THE EFFECT OF AGE ON THE EXPRESSION OF APOPTOSIS BIOMARKERS IN HUMAN SPERMATOZOA |
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Author(s): COLIN A., GOMEZ LOPEZ N., DURAM H., OEHNINGER S., BARROSO G.* |
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* INSTITUTO NACIONAL DE PERINATOLOGIA, MEXICO, MEXICO |
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Abstract:
Background: The negative impact of age on reproductive success has been well demonstrated for women, but age-related changes in the male reproductive system appear to be more subtle. Diminishing testicular function is indicated by a decline in testosterone levels, accompanied by an increase in gonadotropins. Some studies have shown increased age-related numerical and structural sperm chromosomal abnormalities, changes in sperm DNA methylation, and a higher mutational load. Epidemiologic data suggest that increasing male age may be associated with pregnancy loss and with a range of birth defects; the objective was to evaluate the impact of age on the expression of apoptotic biomarkers in human spermatozoa. Materials and Methods: This study was a cross-sectional, prospective study. Healthy volunteers with proven fertility (n=25), stratified by age (range: 20 to 68 years) were subjected to examination of A.M. serum hormone levels [FSH, LH, Prolactin, AMH, T, sex hormone binding globulin (SHBG), and free androgen index (FAI)], basic semen parameters, and assessment of early [plasma membrane translocation of phosphatidylserine (PS)] and late (DNA fragmentation) sperm apoptotic markers by flow cytometry (using Annexin-V binding and Terminal-deoxynucleotidyl-transferase mediated deoxyuridine triphosphate fluorescence nick end labeling, TUNEL, respectively). Main outcome measure: expression of apoptotic markers. Results: The percentage of live sperm cells with plasma membrane translocation of PS (Annexin-V+/PI+) were significantly higher with advancing age (ANOVA, p=0.01). This significant association was further supported by a significant and positive correlation among these two parameters (r= 0.50, p<0.008). Although not significant, there was a clear trend for increased DNA fragmentation in the older groups (r=0.51). The age threshold for these observations appears to be 40 years. Advancing male age was positively correlated with FSH (r= 0.59, p<0.002), SHBG (r=. 63, p<0.01) and FAI (r= -0.67, p<0.01), and negatively correlated with sperm concentration (r= -0.52, p<0.01) and progressive motility (r=-0.67, p<0.01). There was no association between change in hormonal parameters and apoptosis biomarkers. Conclusion: Advancing male age is associated with the expression of early apoptotic markers as evidenced by significantly increased plasma membrane translocation of PS, as well as with a more subtle proportion of sperm carrying DNA fragmentation. This study confirmed that male age is also associated with a decline in sperm concentration and motility.
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Keyword(s): MALE AGE, TUNEL, ANNEXIN, APOPTOSIS, SPERM |
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References:
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Cite:
APA: Copy
COLIN, A., & GOMEZ LOPEZ, N., & DURAM, H., & OEHNINGER, S., & Barroso, G. (2011). ANDROLOGY: THE EFFECT OF AGE ON THE EXPRESSION OF APOPTOSIS BIOMARKERS IN HUMAN SPERMATOZOA. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 5(SUPPLEMENT 1), 25-25. https://www.sid.ir/en/journal/ViewPaper.aspx?id=272047
Vancouver: Copy
COLIN A., GOMEZ LOPEZ N., DURAM H., OEHNINGER S., Barroso G.. ANDROLOGY: THE EFFECT OF AGE ON THE EXPRESSION OF APOPTOSIS BIOMARKERS IN HUMAN SPERMATOZOA. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY. 2011 [cited 2021April15];5(SUPPLEMENT 1):25-25. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=272047
IEEE: Copy
COLIN, A., GOMEZ LOPEZ, N., DURAM, H., OEHNINGER, S., Barroso, G., 2011. ANDROLOGY: THE EFFECT OF AGE ON THE EXPRESSION OF APOPTOSIS BIOMARKERS IN HUMAN SPERMATOZOA. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, [online] 5(SUPPLEMENT 1), pp.25-25. Available at: <https://www.sid.ir/en/journal/ViewPaper.aspx?id=272047>.
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Yearly Visit 27
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