Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   2012 , Volume 48 , Number 1 (189); Page(s) 5 To 6.
 
Paper: 

COMPARISON OF DIFFERENT DETECTION METHODS OF ROSELLINIA NECATRIXIN SOIL

 
 
Author(s):  MOHAMMADI MEYMAND S.M., SHARIF NABI B.*, BAHAR M.
 
* COLLEGE OF AGRICULTURE, ISFAHAN UNIVERSITY OF TECHNOLOGY, ISFAHAN, IRAN
 
Abstract: 

To achieve a simple and sensitive Rosellinia necatrix detection method in soil, three detection methods including soil direct culture method, infected soil in culture media e.g. MEAR, MEAR+ penconazole and MEAS, Nested PCR method and bait twig method were compared. Direct DNA Extraction from soil was done. Two specific primer pairs R2, R8 and R7, R10 in Nested PCR were used and the extracted DNA was purified using PVPP column. Among used methods, the direct culture method from soil was not suitable and wasn’t sensitive for detection. However, bait twig method was capable to detect, the pathogen in soil and root but it is time consuming and laborious method. In conventional PCR, using primer pairs R2-R8, 10 pg ml−1 of fungal DNA was detected; however in Nested PCR using R7-R10 primer pairs, 1fg ml−1 of fungal DNA was detected. Among used methods, the Nested PCR was better in detection of the fungus. Nested PCR method of detection in soil and root is more sensitive and reliable.

 
Keyword(s): SELECTIVE CULTURE MEDIA, BAITING, NESTED PCR, WHITE ROOT ROT AND DETECTION METHODS
 
References: 
  • ندارد
 
  Yearly Visit 82
 
Latest on Blog
Enter SID Blog