In order to study the effect of salinity and silicon application on biomass of shoots and root of fenugreek (Trigonella foenum- graceum L.), a greenhouse experiment was conducted at Research Greenhouse of Ferdowsi University of Mashhad using completely randomized desgin with ten replications. Employed two factors consisted of four levels of salinity using NaCl (0.0, 60, 120 and 180 mM) and two levels of silicon (0.0 and 1.5 mM sodium silicate). Shoot and fruit and root biomass and K content in shoots significantly decreased with increasing salinity, while Na content significantly increased with increasing salinity. Application of silicon imposed a positive effect on preventing high accumulation of Na and increased K content in shoots.