In order to evaluate the effects of fertilization and tillage methods on soil microbial community and sunflower yield, a field experiment was carried out in Sanandaj. The experimental design was a split plot with three replications. Main plots consisted of no tillage (T1), minimum tillage (T2) and conventional tillage (T3). Six methods of fertilization including (N1): 15 t FYM ha-1; (N2): 10 t compost ha-1; (N3): 75 kg triple super phosphate ha-1+150 kg Urea ha-1+50 kg Sulfate potassium; (N4): 10 t FYM ha-1+5 t compost ha-1, (N5): 10 t FYM ha-1+5 t compost ha-1+25 kg triple super phosphate ha-1+75 kg Urea ha-1+25 kg Sulfate potassium and (N6): control were arranged in sub plots. Results showed that the maximum number of bacteria (109.1×106) in dry soil was obtained from the no tillage system. Also, co application of farmyard manure and compost increased bacteria number and phosphatase, urease, catalase, and cellulases activities. All soil enzyme activities decreased significantly with application of chemical fertilizers. The activity of phosphatase (acid and alkaline) and cellulase tended to be higher in the no tillage treatment compared to conventional tillage (51, 27 and 83% respectively). The significantly highest grain yield and oil yield were detected in N5 treatment. The highest grain yield (3701 kg/ha) was produced in minimum tillage system.