Paper Information

Journal:   JOURNAL OF REPRODUCTION AND INFERTILITY   SUMMER 2003 , Volume 4 , Number 3 (15); Page(s) 177 To 184.
 
Paper: 

USE OF SPERMATIDE FOR TREATMENT OF NON-OBSTRUCTIVE AZOOSPERMIC APTIENTS

 
 
Author(s):  AKHOUNDI M.A.*, SEDIGHI M.A., AMIR JANATI N., SADRI ARDEKANI H.
 
* Reproductive Endocrinology & Embryology Dep., Avesina Research Center, Evin, Tehran, Iran
 
Abstract: 

It has been shown that male factor is a common cause of more than half of the infertilities from which 10% are azoospermic patients. Despite multiple testis biopsies, any sperm for microinjection couldn't be obtained in 40% of nonobstructive azoospermic patients. However, recent reports showed successful use of spermatids instead of mature spermatozoa for ICSI which opened a new horizon instead of mature spermatozoa for ICSI which opened a new horizon for spermatids in for these patients. In the present study, 67 nonobstructive azoospermia patients with no sperm in their testis biopsies were enrolled, from whom round spermatilization were extracted from testis and used as male gamete for ovum fertilization. The results including fertilization rate, embryo formation, transfer rate and pregnancy rate were evaluated. Patients were selected based on their history and physical examination, semen analysis, FSH assay and results of multiple testis biopsies with no sperm. Necessary information about use of spermatid as male gamete in microinjection was presented to the patients and their consent were taken. In appropriate time, female cycles were began and simultaneous to egg collection, testis biopsy was performed Mean age of male and female were 32±6.5 and 29.5±8 years, respectively. Mean size of right and left testis were 11±1 and 10.4±0.6 mL, respectively and the mean of FSH level was 21.1±3.2 mIU/mL. Form 760 collected eggs, 537 ovums were injected with spermatids and fertilization rate was 38.2%. Totally, 182 embryos (88.8%) were transferred to the uterus. One chemical pregnancy was observed and documented by ultrasonography, although it was aborted at fourth week. Based on the results of this study, the efficiency of spermatid use in azoospermic patients with no sperm in TEST as a treatment option for their infertility was doubted.

 
Keyword(s): NONOBSTRUCTIVE AZOOSPERMIA, SPERMATID, ICSI, AND TESE
 
References: 
  • ندارد
 
  Yearly Visit 93
 
Latest on Blog
Enter SID Blog