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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   Winter 2004 , Volume 5 , Number 20; Page(s) 158 To 162.
 
Paper: 

A STUDY OF THE CHARACTERISTICS OF LACTOBACILLUS PLANTARUM ISOLATED FROM SAUSAGE IN IRAN

 
 
Author(s):  NOUROUZI JAMILEH*, MIRZAII M.
 
* MICROBILOGY DEPARTMENT, IRAN MEDICAL SCIENCES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Introduction: Lactic acid bacteria are widely used for the fermentation and preservation of dairy and meat products and to improve their aroma and texture. The aim of this study was to screen lactobacillus plantarum isolated from sausage for detections of plasmids, protein bands and phages, to find possible linkage of bacteriocin production to genetic location.
Material and Methods: Two Lactobacillus plantarum with antibacterial activity were isolated from sausage. Bacterial plasmids were isolated by alkali lysis and electrophoresis through agarose gel. Proteins were precipitated from cell - free supernatants by ammonium sulphate and analysed by SDS - PAGE. For detection of phages, mitomycin C of final concentration of 2.5
 mg/ml was used and phages were detected by transmission electron microscopy.
Results: One plasmid of about 4.5 kbp was detected in one Lactobacillus plantarum strain. Two bands of proteins were found on SDS - PAGE. The molecular weight of protein bands of Lacto. Plantarum without plasmid was higher than the protein bands of Lacto. Plantarum with plasmid. A phage was detected on the cell wall of one strain of Lacto. Plantarum; no plasmid was detected in this Lacto. Plantarum. It appears that antibacterial activity is located in the phage of this strain.
Conclusion: the high molecular weigh of proteins with a wide spectrum effect on bacteria may indicate chromosome - coded bacteriocin. The role of phages in lactobacilli could be a factory which inhibit meat product starter cultures or attributed in antimicrobial activity, i.e. antibacterial genes might be on chromosomal phages. Bacteriophages could be a threat to industrial fermentation foods.

 
Keyword(s): LACTOBACILLUS PLANTARUM, PLASMID, SDS - PAGE, PHAGES
 
References: 
 
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