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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   Winter 2004 , Volume 5 , Number 20; Page(s) 128 To 131.
 
Paper: 

IMMUNOGENICITY OF ENTAMOEBA HISTOLYTICA CRUDE AND FRACTIONATED ANTIGENS IN ANIMAL MODEL

 
 
Author(s):  ARIANPOUR N.*, MOHAPATRA T.M.
 
* MICROBIOLOGY DEPARTMENT, MILLITARY MEDICAL UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Introduction: The immunogenicity of crude amoebic antigen and its fractions prepared from Entamoeba histolytica (NIH: 200) was evaluated in experimental animals.
Materials and Methods: Forty two guinea pigs of either sex free from Entamoeba infection and aged around 3 to 4 weeks were randomly divided into 5 groups. The treated groups consisted of 8, 10, 6 and 8 animals served as controls. Crude amoebic extract and its chromatographed fractions were used to immunize the treated animals. All the animals were assessed for immunity status, challenged with Entamoeba trophozoites and subsequently examined for lesions of the caecum and liver.
Results: Of the 8 animals immunized with crude antigen, one had liver abscess and 5 had caecal lesions. None of the 10 animals immunized with fractions I (FI) had hepatic lesions and one had caecal lesions. Both caecal and hepatic lesions were observed in animals immunized with FII & FIII.
Conclusion: Results show that vaccination with the F1 fraction of Entamoeba histolytica provided up to 90% protection against infection. The other fractions and the crude extract provided less protection.

 
Keyword(s): ENTAMOEBA HISTOLYTICA, CRUDE AMOEBIC ANTIGEN, FRACTIONATED AMOEBIC ANTIGENS, PROTECTION, GUINEA PIGS
 
References: 
 
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