ANTI-MUTAGENIC AND PRO-APOPTOTIC EFFECTS OF APIGENIN ON HUMAN CHRONIC LYMPHOCYTIC LEUKEMIA CELLS

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HASHEMI MEHRDAD; NOURI LONG MEHDI; ENTEZARI MALIHEH; NAFISI SHOHREH; NOWROOZII HOSSEIN

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Journal: Acta Medica Iranica Year:2010 | Volume:48 | Issue:5 Page(s): 283-288

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Title

ANTI-MUTAGENIC AND PRO-APOPTOTIC EFFECTS OF APIGENIN ON HUMAN CHRONIC LYMPHOCYTIC LEUKEMIA CELLS

Author(s)

Keywords

ANTIMUTAGENICITY

ANTINEOPLASTIC AGENTS

LEUKEMIA

Abstract

Diet can play a vital role in cancer prevention. Nowadays the scientists are looking for food materials which can potentially prevent the cancer occurrence. The purpose of this research is to examine anti-mutagenic and apoptotic effects of apigenin in human lymphoma cells. In present study human chronic lymphocytic LEUKEMIA (Eheb cell line) were cultured in RPMI 1640 (Sigma), supplemented with 10% fetal calf serum, penicillin-streptomycin, L-glutamine and incubated at 37 oC for 2 days. In addition cancer cell line was treated by and apigenin and cellular vital capacity was determined by MTT assay. Then effect of apigenin in human lymphoma B cells was examined by flow cytometry techniques. The apigenin was subsequently evaluated in terms of anti-mutagenic properties by a standard reverse mutation assay (Ames test). This was performed with histidine auxotroph strain of Salmonella typhimurium (TA100). Thus, it requires histidine from a foreign supply to ensure its growth. The aforementioned strain gives rise to reverted colonies when expose to sodium azide as a carcinogen substance. During MTT assay, human chronic lymphocytic LEUKEMIA revealed to have a meaningful cell death when compared with controls (P<0.01) Apoptosis was induced suitably after 48 hours by flow cytometry assay. In Ames test apigenin prevented the reverted mutations and the hindrance percent of apigenin was 98.17%.These results have revealed apigenin induced apoptosis in human lymphoma B cells in vitro.

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APA: Copy

HASHEMI, MEHRDAD, NOURI LONG, MEHDI, ENTEZARI, MALIHEH, NAFISI, SHOHREH, & NOWROOZII, HOSSEIN. (2010). ANTI-MUTAGENIC AND PRO-APOPTOTIC EFFECTS OF APIGENIN ON HUMAN CHRONIC LYMPHOCYTIC LEUKEMIA CELLS. ACTA MEDICA IRANICA, 48(5), 283-288. SID. https://sid.ir/paper/278444/en

Vancouver: Copy

HASHEMI MEHRDAD, NOURI LONG MEHDI, ENTEZARI MALIHEH, NAFISI SHOHREH, NOWROOZII HOSSEIN. ANTI-MUTAGENIC AND PRO-APOPTOTIC EFFECTS OF APIGENIN ON HUMAN CHRONIC LYMPHOCYTIC LEUKEMIA CELLS. ACTA MEDICA IRANICA[Internet]. 2010;48(5):283-288. Available from: https://sid.ir/paper/278444/en

IEEE: Copy

MEHRDAD HASHEMI, MEHDI NOURI LONG, MALIHEH ENTEZARI, SHOHREH NAFISI, and HOSSEIN NOWROOZII, “ANTI-MUTAGENIC AND PRO-APOPTOTIC EFFECTS OF APIGENIN ON HUMAN CHRONIC LYMPHOCYTIC LEUKEMIA CELLS,” ACTA MEDICA IRANICA, vol. 48, no. 5, pp. 283–288, 2010, [Online]. Available: https://sid.ir/paper/278444/en

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