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Paper Information

Journal:   JOURNAL OF VETERINARY RESEARCH   2011 , Volume 66 , Number 1; Page(s) 61 To 64.
 
Paper: 

DETERMINATION OF ANTIGENIC PROTEIN OF DICROCOELIUM DENDRITICUM IN NATURALLY INFECTED SHEEP

 
 
Author(s):  FAKOUR SH., MESHGI B.*
 
* DEPARTMENT OF CLINICAL SCIENCES, FACULTY OF VETERINARY MEDICINE, AZAD ISLAMIC UNIVERSITY OF SANANDAJ, SANANDAJ, IRAN
 
Abstract: 

Dicrocoeliasis is an important livestock disease caused by digenean trematode namely Dicrocoelium dendriticum. The aim of the present study was to identify somatic and metabolic antigens of adult D.dendriticum in naturally infected sheep. Adult parasites collected from the liver of naturally infected sheep, were washed in cold phosphate buffer saline (PBS, pH=7.4) and stored at -20°c until analysis. Antigen used for the detection of antibody included somatic and metabolic of mature trematode. Somatic and excretory-secretory antigens prepared with haemogenization and incubation of adult helminths, respectively. Electrophoretic patterns of excretory secretory and somatic antigens of D.dendriticum were revealed by sodium dodecyl sulphate polyacrylamid gel electrophoresis (SDS-PAGE) and westernblotting using sera from naturally harbored sheep. In western blot analysis of antigens D.dendriticum demonstrate 1 major antigenic polypeptide 130 kDa in both somatic and metabolic antigens and 6 protein bands ranging from 25 to 60 kDa in excretory-secretory antigens which were recognized by serum of sheep naturally infected. Our findings showed that the 130 kDa molecular weight polypeptide could be used as specific antigen for the immunodiagnosis of sheep dicrocoeliasis.

 
Keyword(s): DICROCOELIUM DENDRITICUM, SPECIFIC ANTIGEN, SHEEP
 
 
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APA: Copy

FAKOUR, S., & MESHGI, B. (2011). DETERMINATION OF ANTIGENIC PROTEIN OF DICROCOELIUM DENDRITICUM IN NATURALLY INFECTED SHEEP. JOURNAL OF VETERINARY RESEARCH, 66(1), 61-64. https://www.sid.ir/en/journal/ViewPaper.aspx?id=194538



Vancouver: Copy

FAKOUR SH., MESHGI B.. DETERMINATION OF ANTIGENIC PROTEIN OF DICROCOELIUM DENDRITICUM IN NATURALLY INFECTED SHEEP. JOURNAL OF VETERINARY RESEARCH. 2011 [cited 2021July30];66(1):61-64. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=194538



IEEE: Copy

FAKOUR, S., MESHGI, B., 2011. DETERMINATION OF ANTIGENIC PROTEIN OF DICROCOELIUM DENDRITICUM IN NATURALLY INFECTED SHEEP. JOURNAL OF VETERINARY RESEARCH, [online] 66(1), pp.61-64. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=194538.



 
 
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