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Paper Information

Journal:   SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL   SUMMER 2010 , Volume 6 , Number 2 (27); Page(s) 49 To 53.
 
Paper: 

DRUG RESISTANCE STUDY OF SALMONELLA SPP. ISOLATED FROM NATIVE EGGS

 
 
Author(s):  JAHANTIGH M.*
 
* DEPARTMENT OF CLINICAL SCIENCES, FACULTY OF VETERINARY MEDICINE, UNIVERSITY OF ZABOL, IRAN
 
Abstract: 
Salmonellosis is a group of diseases caused by the members of the genus Salmonella. Poultry are the main source of Salmonella spp. and amongst the animal species, salmonellae are mainly reported from poultry and their products. Avian Salmonella are important as both a cause of clinical disease in poultry and as a source of food-borne transmission of disease to humans. In order to study the native eggs contamination to Salmonella spp. and their drug resistance pattern, the eggs were collected from Zabol region of Iran and transferred to the Microbiology laboratory. In the laboratory, the eggs shells were cleaned and disinfected with 96% ethanol. The shells were crushed and the contents of 5 eggs were pooled in a beaker glass and incubated at 37 oC for 24h. One swab contaminated by the incubated pooled eggs and inoculated into Selenite-F broth and after incubation at 37 oC was subcultured on Salmonella-Shigella agar. The suspected colonies on Salmonella-Shigella agar were stained by gram method and pure cultures prepared from the suspected colonies. The suspected colonies were inoculated into lysine decarboxylase broth and TSI agar. The colonies with typical Salmonella reactions were examined by PCR using specific primers targeting the invA, sefA and pefA genes for Salmonella spp., S. enteritidis and S. typhimurium respectively. The results of this study showed that seven samples (at least 4% of the eggs) had S. enteritidis contamination. Study of resistance to antimicrobial agents of isolated Salmonellae showed the most susceptibility to Norfloxacin (100% sensitivity of the isolates).
 
Keyword(s): SALMONELLA SPP., BACTERIAL CULTURE, DRUG RESISTANCE
 
References: 
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  Persian Abstract Yearly Visit 66
 
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