Paper Information

Journal:   PHYSIOLOGY AND PHARMACOLOGY   FALL 2009 , Volume 13 , Number 3; Page(s) 263 To 270.
 
Paper: 

NEUROPROTECTIVE EFFECT OF NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT ON SERUM/GLUCOSE DEPRIVATION INDUCED PC12 CELLS DEATH

 
 
Author(s):  TAYARANI NAJARAN ZAHRA, SADEGHNIA HAMID REZA, ASGHARI MOZHGAN, MOUSAVI SEYED HADI*
 
* DEPARTMENT OF BIOCHEMISTRY, PAYAME NOOR UNIVERSITY
 
Abstract: 
Introduction: The Serum/Glucose deprivation -induced cell death in cultured PC12 cells represents a useful in vitro model for the study of brain ischemia and neurodegenerative disorders. Nigella sativa L. has been known as a source of antioxidants. To elucidate the neuroprotective actions of N. sativa extract in vitro, we studied the effect of N. sativa extract on cultured PC12 cells under serum/glucose deprivation conditions.
Methods: PC12 cells were cultured in DMEM medium containing 10% (v/v) fetal bovine serum, 100 units/ml penicillin, and 100
mg/ml streptomycin. Cells were seeded overnight and then deprived of serum/glucose for 6 and 18 h.
Cells were pretreated with different concentrations of N. sativa extract (7.81-250
mg/ml). Cell viability was quantitated by MTT assay. Intracellular ROS production was measured by flow cytometry using 2', 7’-Dichlorofluorescin diacetate (DCF-DA).
Results: Depriving the PC-12 cells of serum/glucose caused prominent cell toxicity at least after 6 and 18 h.
Pretreatment of PC12 cells with N. sativa (7.81-250
mg/ml) could reduce serum/glucose deprivation-induced cytotoicity in PC12 cells after 18 h. The experimental results suggest that N. sativa extract protects the PC12 cells against Serum/Glucose deprivation-induced cytotoxicity.
Conclusion: Our findings might raise a possibility of potential therapeutic application of N. sativa extract for preventing and treating cerebral ischemic and neurodegenerative diseases.
 
Keyword(s): PC12, SERUM/GLUCOSE FREE, TOXICITY, NIGELLA SATIVA
 
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