Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   FALL 2009 , Volume 4 , Number 3; Page(s) 55 To 62.
 
Paper: 

EVALUATION OF GENETIC DIVERSITY IN WHEAT GERMPLASM USING RAPD MARKERS

 
 
Author(s):  DASHTI H.*, NAGHAVI M.R., SHAHNEJATBUSHEHRI A.A., SHIRANI H.
 
* DEPARTMENT OF AGRONOMY AND PLANT BREEDING, FACULTY OF AGRICULTURE, VALI-E-ASR UNIVERSITY OF RAFSANJAN
 
Abstract: 

I dentification of genomic homologies provides some important tools for improving conservation and management of plant genetic resources. In order to evaluate genetic diversity in wheat germplasm, 30 samples from bread wheat, durum wheat and diploid wild relatives (AA and DD) were randomly selected and analysed using 11 ten-mer RAPD primers. These primers amplified different parts of the genome as a total of 105 loci with 953 bands were obtained. C and E primers amplified higher loci compared to other primers, while primer UBC64 showed the highest amount of bands. All used primers were able to determine genetic differences among genotypes. Two special bands were introduced for DDdiploids by F primer. Cluster analysis using UPGMA and Jaccards’ similarity coefficient revealed that the nearest samples were in 0.9 similarity, while the farther samples were in 0.5 similarity. At 0.7 similarity, evaluated samples clustered in 4 and at 0.65 similarity clustered in 2 main groups. This research showed that the accessions under study have not high genetic diversity the results of PCoA, approximately was in agreement with cluster analysis.

 
Keyword(s): GENETIC DIVERSITY, GERMPLASM, WHEAT, RAPD, CLUSTER ANALYSIS
 
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