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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   FALL 2009 , Volume 4 , Number 3; Page(s) 63 To 70.
 
Paper: 

THE EFFECTS OF GENOTYPE AND CYTOKININS ON EMBRYOGENIC CALLUS PRODUCTION OF DATE PALM (PHOENIX DACTYLIFERA L.)

 
 
Author(s):  DANIYAIE A., MOUSAVI AMIR*, FARAHANI F., DEHSARA B., ZAEFIZADEH M.
 
* NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY, TEHRAN, IRAN
 
Abstract: 

In order to optimize embryogenic calli production in date palm, meristematic tissues were excised from 2-3 years old offshoots of Zahedi, Kabkab and Dayri genotypes and cultured on MS medium containing 100 mg/l 2,4-D, 3 g/l activated charcoal, 30 g/l sucrose supplemented with different concentrations of zeatin and TDZ as cytokinin resources. The samples were incubated at 25±2°C in the darkness. Initial calli were generated from explants cultured in various treatments which were able to produce embryogenic callus after 6-8 weeks of sub culturing in the same medium. The best callus production was observed in 0.5 and 3.0 mg/l of zeatin with respect to Zahedi and Kabkab cultivars and 0.1 mg/l of TDZ for Dayri. Meanwhile, the shortest callus generation time was correspondent to Kabkab, Zahedi and Dayri genotypes, respectively.

 
Keyword(s): CALLUS, DATE PALM, SOMATIC EMBRYOGENESIS, TISSUE CULTURE
 
References: 
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