Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   HEPATITIS MONTHLY   APRIL 2011 , Volume 11 , Number 4 (33); Page(s) 255 To 262.
 
Paper: 

THE DIFFERENCES IN GENE EXPRESSION PROFILE INDUCED BY GENOTYPE 1B HEPATITIS C VIRUS CORE ISOLATED FROM LIVER TUMOR AND ADJACENT NON-TU-MORAL TISSUE

 
 
Author(s):  YAN XUE BING*, CHEN ZHI, BRECHOT CHRISTIAN
 
* DEPARTMENT OF INFECTIOUS DISEASES, THE FIRST AFFILIATED HOSPITAL OF XUZHOU MEDICAL COLLEGE, XUZHOU 221002, JIANGSU PROVINCE, CHINA
 
Abstract: 

Background: Core protein of the hepatitis C virus (HCV) has an important role in HCV self-replication, pathogenesis and carcinogenesis.
Objectives: To identify the effect of core proteins from different quasispecies of HCV genotype 1b expressed in a HepG2 cell line on human gene expression profiles.
Materials and Methods: Core protein eukrocytic expression plasmids (pEGFP-N1) containing different quasispecie core protein genes of genotypes 1b HCV derived from of HCV-related hepatocellular carcinoma (HCC) tumoral tissue (T) and non-tumoral tissue (NT) were constructed and then transfected to HepG2 cell line. The gene expression spectrum in the cell expression core proteins from T and NT were compared with those in the control by Affymetrix human genome HG-U 133 plus 2.0 microarray.
Results: Different gene expression profiles were acquired between HepG2 expressing core proteins derived from T and NT tissues. Both core proteins caused the modulation of several genes that are up/down-regulated as compared to control, including genes involved in oncogenesis, signal transduction, cell apoptosis, and cell growth cycle regulation. Surprisingly, only one gene-CSNK1A1-was up-regulated by both T and NT core variants.
Conclusions: Core proteins isolated from tumoral or non-tumoral nodules mediate expression of different cellular genes suggesting that variants isolated from different quasispecies may have different biological effects.

 
Keyword(s): HEPATITIS C VIRUS, VIRAL CORE PROTEINS, GENOTYPES, MICROARRAY ANALYSIS, GENE EXPRESSION
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 103
 
Latest on Blog
Enter SID Blog