Paper Information

Journal:   VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)   WINTER 2010 , Volume 3 , Number 4 (12); Page(s) 673 To 681.
 
Paper: 

DETECTION OF MYCOPLASMA SYNOVIAE IN CLINICAL SAMPLES BY VIHA-PCR METHOD

 
 
Author(s):  ANSARI H., POURBAKHSH S.A.*, SHEIKHI N., BOZORGMEHRI FARD M.H., ASHTARI A.
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF SPECIALIZED VETERINARY SCIENCES, ISLAMIC AZAD UNIVERSITY, SCIENCE & RESEARCH BRANCH, TEHRAN, IRAN
 
Abstract: 

As one of the major pathogens of avian species, Mycoplasma Synoviae causes significant economic losses to the poultry industry. The main purpose of this study was to detect Mycoplasma Synoviae in clinical samples using the VIhA-PCR method. For serological screening test, 373 serum samples were collected from 25 breeder farms and rapid serum agglutination test conducted which revealed that 143 samples equivalent to 19 breeder farms were positive. For VIhA-PCR assay, 20 of the previously mentioned breeder farms were selected and sterile swab were collected from the palatine cleft, trachea, air sacs and lungs. Three swabs from 3 birds were placed in a test tube containing 1 ml of PBS and transferred to the laboratory for PCR test. Specific primers for VIhA gene were employed in this study. The PCR product from specific primers showed 350-400 bp for all field isolated on electrophoresis gel in 8 farms. VIhA-PCR with high sensitivity could be employed in definitive diagnosis of Mycoplasma Synoviae infection in the laboratory.

 
Keyword(s): MYCOPLASMA SYNOVIAE, CLINICAL SAMPLES, PCR, VIHA GENE
 
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