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Paper Information

Journal:   NEW FINDINGS IN AGRICULTURE   FALL 2007 , Volume 2 , Number 1; Page(s) 45 To 57.
 
Paper: 

ISOLATION OF β-1 AND 3-GLOCONASE FROM MEDICAGO TRUNCATULA, EXSPRESS THEM IN E.COLI BL21 AND INVESTIGATION OF THEIR FUNGICIDE

 
 
Author(s):  NABAVI N.*, NAJMI B., RASOOLI M.
 
* 
 
Abstract: 
Pathogenesis related proteins are compounds that produced by response to pathogens or environmental stress. Up to now numerous kind of b-1 and 3-Glucanase are identified in various plants and thein fungicide effect were tested. The aim of this research was isolation, over-expression and analysis of physiological activities of b-1 and 3-Glucanase (PR-2) from Medicago truncatula. In this case by Medicago sativa and Pisum sativum b-1 and 3-Glucanase gene alignment on Medicago truncatula genome three open reading frame with%50 similarity by having start and stop codon were identified and isolated that fragments were cloned in PET21c vector and expressed in E.coli BL21. SDS PAGE technique reveal that proteins were produce as an inclusion bodies. We also test their fungicide effect after folding them to achieve their natural structure on Alternaria alternaria and Fusarium graminearum. The result were significant (P<0.05) by SAS system analysis.
 
Keyword(s): β-1AND 3-GLUCANASE, MEDICAGO TRUNCATULA, PATHOGENESIS RELATED PROTEINS
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

NABAVI, N., & NAJMI, B., & RASOOLI, M. (2007). ISOLATION OF β-1 AND 3-GLOCONASE FROM MEDICAGO TRUNCATULA, EXSPRESS THEM IN E.COLI BL21 AND INVESTIGATION OF THEIR FUNGICIDE. NEW FINDINGS IN AGRICULTURE, 2(1), 45-57. https://www.sid.ir/en/journal/ViewPaper.aspx?id=193551



Vancouver: Copy

NABAVI N., NAJMI B., RASOOLI M.. ISOLATION OF β-1 AND 3-GLOCONASE FROM MEDICAGO TRUNCATULA, EXSPRESS THEM IN E.COLI BL21 AND INVESTIGATION OF THEIR FUNGICIDE. NEW FINDINGS IN AGRICULTURE. 2007 [cited 2021July30];2(1):45-57. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=193551



IEEE: Copy

NABAVI, N., NAJMI, B., RASOOLI, M., 2007. ISOLATION OF β-1 AND 3-GLOCONASE FROM MEDICAGO TRUNCATULA, EXSPRESS THEM IN E.COLI BL21 AND INVESTIGATION OF THEIR FUNGICIDE. NEW FINDINGS IN AGRICULTURE, [online] 2(1), pp.45-57. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=193551.



 
 
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