Paper Information

Journal:   BIOLOGY JOURNAL   SPRING 2009 , Volume 4 , Number 1; Page(s) 2 To 8.
 
Paper: 

THE STUDY OF ULTRASTRUCTURE FEATURES, ALLERGENICITY AND INFLUENCE OF AIR POLLUTION ON ALLERGENICITY OF MATURE POLLENS IN CERCIS SILIQUASTRUN

 
 
Author(s):  CHALABIAN FIROOZEH*, MANSOURI MARYAM, SHARIFNIA FARIBA
 
* BIOLOGY DEPARTMENT, ISLAMIC AZAD UNIVERSITY, NORTH- TEHRAN BRANCH
 
Abstract: 
Cercis siliquastrum that belongs to caesalpiniaceae family is an ornamental plant. The aim of this research is study of ultrastructure features, allergenicity and influence of air pollution on allergenicity of mature pollens in cercis siliquastrun. S.E.M showed that pollens of this plant are oval, Tricoplate that average size is 20mm. Exine thornies is reticulate. Because that the most important part of allergenicity of pollen is protein, we studied quantity and quality of pollen’s protein. In quantity study with Bradford method protein content is more than 1000mgm1-1. In comparision of clean and polluted pollen extraction has’nt observed significant differences. In electrophorase study that has done with SDS-PAGE method, protein bands observed with molecular weights of 14, 18, 37, 54, 65 and 110KD. Pollen extraction injected to Guinea pigs in 3 groups: control, treatmented with clean pollen extraction and treatmented with polluted pollen extraction. The amount of total IgE with ELISA method showed that IgE changed from 3.481um1-1 in control group to 6.241um1-1in treatmented group with clean pollen extraction and 6.381um1-1 in treatmented with polluted pollen extraction Eosionophile percentage changed from 1% in control group to 2.14% in treatmented group with clean pollen extraction and 2.2% in treatmented group with polluted pollen extraction.
There was significant differences between control group and treatment group. Because of this results, Pollens of Cercis siliquastrum are allergen.
 
Keyword(s): ALLERGENCITY, POLLEN STRUCTURE, CIRCIS SILIQUASTRUM, CAESALPINIACEAE FAMILY
 
References: 
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