Paper Information

Journal:   VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)   SUMMER 2009 , Volume 3 , Number 2 (10); Page(s) 451 To 457.
 
Paper: 

IMMUNOHISTOCHEMICAL STUDY OF β--CATENIN IN EXPERIMENTAL COLON CARCINOMA OF RAT

 
Author(s):  DOUSTAR Y.*, MOHAJERI D., FATHI AZAR F., NAMVARAN A.
 
* DEPARTMENT OF PATHOBIOLOGY, FACULTY OF VETERINARY MEDICINE, ISLAMIC AZAD UNIVERSITY, TABRIZ BRANCH, TABRIZ, IRAN
 
Abstract: 

Colorectal adenocarcinoma is one of the most prevalent and treatable malignancies of the gastrointestinal tract. Recent studies on colorectal neoplasia indicate b-catenin gene mutation and its intranuclear accumulation inside hyperplastic cells. Therefore, b-catenin may be an important prognosis and diagnostic index of this disease. The aim of this study is to evaluate the nuclear beta-catenin expression in hyperplastic cells of colon following treatment with 1,2-dimethylhydrazine. In this study, 56 wistar male rats with the age of 12 weeks and body weight of 200-300g were selected and randomly allocated into two equal treatment and control groups. In the treatment group, two subcutaneous injections of 1,2-dimethylhydrazine every week at a dose rate of 40 mg/kg were used for 4 weeks to induce colon carcinoma. The control group was given normal saline solution similarly. After 8 weeks, tissues samples of distal colon were collected from both groups for preparation of tissue sections and immunohistochemistry. Immunohistopathological study of samples revealed that nuclear b-catenin expression in the treatment group was significantly higher than the control group (p<0.01). The study indicates that the expression of nuclear beta-catenin can increase in hyperplasia and neoplasia of colon.

 
Keyword(s): COLON, CARCINOMA, β-CATENIN, IMMUNOHISTOCHEMISTRY
 
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