Paper Information

Journal:   JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES   JUNE-JULY 2010 , Volume 18 , Number 2 (70); Page(s) 50 To 56.
 
Paper: 

STUDY OF THE EXPRESSION OF SURVIVIN & ITS SPLICE VARIANTS; ∆EX3, 2B AND 3B AS DIAGNOSTIC MOLECULAR MARKERS IN BREAST CANCER

 
 
Author(s):  HOSSEINPOUR FEYZI M.A., MONIRI S., HESARI J., BABAEI E., MONTAZERI V., HALIMI M.
 
* 
 
Abstract: 

Introduction: Survivin is a new member of the Inhibitor Apotosis Protein family (IAP) which plays an important role in the regulation of both cell cycle and apoptosis. Its distinct expression in tumor cells as compared to normal adult cells introduces Survivin as the fourth transcriptom demonstrated in tumors. Breast cancer is the most common malignancy among women and scientist`s efforts to classify it has lead to various molecular subtypes and controversial results. Because of the high prevalence of these tumors and lack of suitable molecular markers for diagnosis and prognosis, there are ongoing efforts to find molecular markers which can distinguish nontumoral from tumor tissues. In this study we evaluate the potential usefulness of Survivin and its splice variants DEx3, 2b and 3b as molecular markers in breast cancer.
Methods: We studied 18 tumor and 17 non tumor adjacent tissues. Transcription levels were measured by Semiquantitative Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and normalized by
b2m as an internal control. Results: 1)Survivin and its splice variants; Dex3, 2b and 3b showed differentially higher expression levels in tumors than adjacent normal tissues. 2) The expression levels of Survivin, Survivin-DEx3 and Survivin-3b were significantly correlated with the type of tumors. 3) Survivin-2b was expressed in a few samples. 4) Survivin-3b was detected only in tumor samples. Also, our results showed that DEx3 variant can be introduced as a dominant expressed variant in breast cancer.
Conclusion: Our data indicated that the expression of Survivin, Survivin
DEx3 and especially, Survivin-3b were correlated with cancerous nature of tumors and Survivin-DEx3 was the most common expressed variant in breast carcinomas. These results besides confirming the potential usefulness of Survivin and its splice variants as molecular markers in breast cancer, demonstrated the role of the gene and its splice variants, especially 3b in these lesions that enables distinguishing normal from tumor tissues. Therefore, evaluation of the expression of Survivin and its splice variants might be used as markers to stratify breast cancer patients for more optimal treatment modalities or it could be a promising new target for therapy.

 
Keyword(s): SURVIVIN, SPLICE VARIANT, SEMIQUANTITATIVE RT-PCR, BREAST CANCER
 
References: 
  • ندارد
 
  Yearly Visit 31
 
Latest on Blog
Enter SID Blog