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Paper Information

Journal:   JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS)   JANUARY 2011 , Volume 16 , Number 1; Page(s) 50 To 55.
 
Paper: 

ANALYSIS OF MITOCHONDRIAL ND1 GENE IN HUMAN COLORECTAL CANCER

 
 
Author(s):  AKOUCHEKIAN MANSOUREH, HOUSHMAND MASSOUD*, HOSSEINI AKBARI MOHAMMAD HASSAN, KAMALIDEHGHAN BEHNAM, DEHGHAN MASOUMEH
 
* DEPARTMENT OF MEDICAL GENETICS, NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY (NIGEB), TEHRAN, IRAN
 
Abstract: 

BACKGROUND: Colorectal cancer as a mortal disease affected both sexes of all ethnic and racial human groups. Former studies have indicated some mutations in the mitochondrial DNA (mtDNA) in different human cancers. Complex I NADH has the most subunits encoded by mtDNA. For a better understanding of the mtDNA abnormality in colorectal cancer some genes of this complex is screened for existence of mutations.
METHODS: One of the main regions of the mtDNA encoding protein was screened by PCR-RFLP followed by DNA sequencing. The obtained sequences were aligned with the revised Cambridge Reference Sequence (rCRS). Each alteration recorded as single nucleotide polymorphisms (SNPs), deletions or insertions.
RESULTS: Eight mutations were found in 15 samples out of 30 studied populations and no mutation detected in other 15 samples. Among these 15 mutated samples, 7 different mutations were found in 7 patients, that means one mutation per patient and the 8th mutation (T4216C) was common in the rest of 8 samples; in other words T4216C mutation in 27% of tested samples was identified (8 patients out of 30 patients). The existence of T4216C mutation was found to be significantly different (p
£ 0.05) between tumoral patient's tissue and adjacent normal tissue.
CONCLUSIONS: Results showed that a high frequency of somatic alterations of mtDNA occurs during the carcinogenesis and/or the progression of colorectal cancer. Based on the mtDNA mutation pattern observed in this study and other previously studies it is believed that looking for somatic mutations in mtDNA would be one of the diagnostic values in early detection of cancer.

 
Keyword(s): DNA, MITOCHONDRIAL, COLORECTAL NEOPLASMS, ELECTRON TRANSPORT COMPLEX I, MT-ND1 PROTEIN, HUMAN, OXIDATIVE PHOSPHORYLATION, REACTIVE OXYGEN SPECIES
 
 
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APA: Copy

AKOUCHEKIAN, M., & HOUSHMAND, M., & HOSSEINI AKBARI, M., & KAMALIDEHGHAN, B., & DEHGHAN, M. (2011). ANALYSIS OF MITOCHONDRIAL ND1 GENE IN HUMAN COLORECTAL CANCER. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), 16(1), 50-55. https://www.sid.ir/en/journal/ViewPaper.aspx?id=192742



Vancouver: Copy

AKOUCHEKIAN MANSOUREH, HOUSHMAND MASSOUD, HOSSEINI AKBARI MOHAMMAD HASSAN, KAMALIDEHGHAN BEHNAM, DEHGHAN MASOUMEH. ANALYSIS OF MITOCHONDRIAL ND1 GENE IN HUMAN COLORECTAL CANCER. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS). 2011 [cited 2021July27];16(1):50-55. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=192742



IEEE: Copy

AKOUCHEKIAN, M., HOUSHMAND, M., HOSSEINI AKBARI, M., KAMALIDEHGHAN, B., DEHGHAN, M., 2011. ANALYSIS OF MITOCHONDRIAL ND1 GENE IN HUMAN COLORECTAL CANCER. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), [online] 16(1), pp.50-55. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=192742.



 
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