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Paper Information

Journal:   IRANIAN JOURNAL OF FIELD CROPS RESEARCH   2009 , Volume 7 , Number 2; Page(s) 637 To 646.
 
Paper: 

FUNCTIONAL CLONING OF RESISTANT GENES AGAINST LATE BLIGHT IN SOLANUM DEMISSUM GENOTYPE

 
 
Author(s):  MORTAZAVIAN S.M.M., ZALI A.A., SHAH NEJAT BUSHEHRI A.A., BIHAMTA M.R., VAN DER VOSSEN E.A.G., VOSSEN J.
 
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Abstract: 

Demissum differentials are important for pathogen isolates identification. Recent studies show Ma.R9 contains R2 and R3 or homologues of the genes. This genotype has a high resistance against different isolates in the world. Candidate gene approach applied to isolate and cloning of these homologues in this genotype. Phylogenetic clades drawn and expression assay was done by coinfiltration in N. benthamiana. Result revealed this genotype contain R3a and also functional R2 like gene. Alignment of R2 gene against R2-like in amino acids level showed completely conserved residues in LRR region. Presence of more than one gene could be the reason of resistance against different isolates. These results confirm high efficiency of candidate cloning approach and effector genomics as new approaches.

 
Keyword(s): CONTRIBUTION FROM COLLEGE OF AGRICULTURE, UNIVERSITY OF TEHRAN AND PRI, WAGENINGEN, THE NETHERLANDS
 
References: 
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