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Paper Information

Journal:   THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)   SPRING 2011 , Volume 8 , Number 1 (30); Page(s) 42 To 51.
 
Paper: 

Development Of Nasba-Elisa Technique For Detection Of Hiv-1 Rna

 
 
Author(s):  Shahrabi M., Forouzandeh M.*, Sabahi F., Paryan M.
 
* DEPARTMENT OF MEDICAL BIOTECHNOLOGY, FACULTY OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 
Background and Objectives: Viral RNA is the first blood marker which appears in HIV-1 infection. RT-PCR and NASBA are the commonly used techniques for amplification of RNA. NASBA technique provides more advantages over RT-PCR. In this study, an NASBA assay combined with an easy, sensitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of HIV-1 RNA.Materials and Methods: 11-dig-UTP was added to an NASBA reaction mixture and the RNA amplicons were labeled. Then, the dig-labeled NASBA products hybridized to a biotinylated specific probe in hybridization solution buffer and the hybrids were transferred to a streptoavidin-coated plate. After several washing processes and emission of nonspecific products, the final detection of the captured RNA was done by addition of anti-dig antibody-enzyme conjugate and the substrate.Results: The results demonstrated that, NASBA is an efficient method for amplification of the target genome. Detection of NASBA products by agarose gel electrophoresis showed a unique 176 bp band corresponding to the specific target. For the detection of NASBA products, an ELISA assay was performed; using 0.01 PM probe, the OD of 1.049 ± 0.11 was obtained. Conclusions: In this study, by using suitable primers and probe a highly sensitive and specific NASBAELISA method for detection of HIV-1 developed.
 
Keyword(s): NASBA, ELISA, IMMUNOASSAY, HIV-1
 
 
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APA: Copy

SHAHRABI, M., & FOROUZANDEH, M., & SABAHI, F., & PARYAN, M. (2011). DEVELOPMENT OF NASBA-ELISA TECHNIQUE FOR DETECTION OF HIV-1 RNA. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 8(1 (30)), 42-51. https://www.sid.ir/en/journal/ViewPaper.aspx?id=191694



Vancouver: Copy

SHAHRABI M., FOROUZANDEH M., SABAHI F., PARYAN M.. DEVELOPMENT OF NASBA-ELISA TECHNIQUE FOR DETECTION OF HIV-1 RNA. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON). 2011 [cited 2022January26];8(1 (30)):42-51. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=191694



IEEE: Copy

SHAHRABI, M., FOROUZANDEH, M., SABAHI, F., PARYAN, M., 2011. DEVELOPMENT OF NASBA-ELISA TECHNIQUE FOR DETECTION OF HIV-1 RNA. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), [online] 8(1 (30)), pp.42-51. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=191694.



 
 
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