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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   MARCH-APRIL 2011 , Volume 14 , Number 2 (49); Page(s) 132 To 136.
 
Paper: 

BIOFILM FORMATION AND DETECTION OF ICAAB GENES IN CLINICAL ISOLATES OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS

 
 
Author(s):  EFTEKHAR FERESHTEH*, DADAEI TARANEH
 
* MICROBIOLOGY DEPARTMENT, FACULTY OF BIOLOGICAL SCIENCES, SHAHID BEHESHTI UNIVERSITY, G.C., EVIN, TEHRAN, IRAN
 
Abstract: 
Objective(s)
Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less well described in S. aureus. We studied biofilm formation in clinical isolates of MRSA in relation to the presence of the ica operon.
Materials and Methods
Forty five MRSA were studied for biofilm formation by colony morphology on Congo red agar (CRA) and the microtitre plate assay (MtP). Presence of the ica genes was detected by PCR and specific primers.
Results
The results showed that 53.3% of the isolates had the potential to form biofilm by colony morphology of which, 75% carried the ica operon. Weak biofilm production was observed in the MtP assay by 57.8%, of which 53.8% harbored the ica operon. However, about 70% of biofilm non-producers also carried the ica operon.
Conclusion
Overall, there was no agreement between the icaAB gene carriage and biofilm phenotype by either of the two phenotypic methods. However, 91% of biofilm formers on CRA also produced biofilm in the MtP assay.
 
Keyword(s): BIOFILM FORMATION, ICAAB, MRSA, STAPHYLOCOCCUS AUREUS
 
 
References: 
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Citations: 
 
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APA: Copy

EFTEKHAR, F., & DADAEI, T. (2011). BIOFILM FORMATION AND DETECTION OF ICAAB GENES IN CLINICAL ISOLATES OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 14(2 (49)), 132-136. https://www.sid.ir/en/journal/ViewPaper.aspx?id=191293



Vancouver: Copy

EFTEKHAR FERESHTEH, DADAEI TARANEH. BIOFILM FORMATION AND DETECTION OF ICAAB GENES IN CLINICAL ISOLATES OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2011 [cited 2021June22];14(2 (49)):132-136. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=191293



IEEE: Copy

EFTEKHAR, F., DADAEI, T., 2011. BIOFILM FORMATION AND DETECTION OF ICAAB GENES IN CLINICAL ISOLATES OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 14(2 (49)), pp.132-136. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=191293.



 
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