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Paper Information

Journal:   JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ)   FALL 2010-WINTER 2011 , Volume 20/3 , Number 2; Page(s) 13 To 22.
 
Paper: 

OPTIMIZATION OF PECTINLYASE PRODUCTION FROM DATE PULP BY ASPERGILLUS NIGER USING RESPONSE SURFACE METHODOLOGY

 
 
Author(s):  FARBEH F., REZAZAD BARI M.*, ALIZADEH KHALEDABAD M.
 
* DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY, FACULTY OF AGRICULTURE, UNIVERSITY OF URMIA, IRAN
 
Abstract: 

In this study a two-step approach was applied in order to investigate the effect of seven factors including: concentration of ammonium sulphate, potassium dihydrogen phosphate, date pulp, pH, inoculums size, agitation speed and fermentation time on the pectinlyase production by Aspergillus niger. At first step, the effects of these variables were studied using fractional factorial design. According to obtained results three factors, pH, ammonium sulphate concentration and fermentation time were considered for further study. Second step was carried out by using central composite design (CCD) in order to find optimum conditions of pH, ammonium sulphate concentration and fermentation time. After analysis of CCD, optimum condition for maximization of pectinlyase activity determined as follow: ammonium sulphate concentration 0.35%, fermentation time, 73.93 hours and pH, 4.82. Enzyme activity at this optimum condition was 1.38 U. ml -1.

 
Keyword(s): PECTINLYASE, ASPERGILLUS NIGER, OPTIMIZATION, DATE PULP
 
References: 
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