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Paper Information

Journal:   JOURNAL OF MICROBIAL BIOTECHNOLOGY   SUMMER 2009 , Volume 1 , Number 1; Page(s) 23 To 27.
 
Paper: 

ISOLATION, SCREENING AND IDENTIFICATION OF BI-FUNCTIONAL PUTA FLAVOENZYME PRODUCING PSEUDOMONAS FLUORESENSE FROM SOIL

 
 
Author(s):  SHAHBAZ MOHAMMADI H.*, OMIDINIA E.
 
* DEPARTMENT OF BIOCHEMISTRY, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 

Background and Objective: The bifunctional proline utilization A (PutA) contains praline dehydrogenase and D-pyrroline-5-carboxylate dehydrogenase domains and plays an important role in the metabolic pathway of proline to glutamate. The aim of this research was to isolate and identify the PutA falvoenzyme producing bacteria with appropriate kinetic properties.
Materials and Methods: Needed soil samples were collected from different places of Tehran city including Darakeh, Darband, Farahzad and Damavand. Isolation and screening of falvoenzyme producing microorganisms was performed by enrichment culture technique in proline specific culture medium and enzyme activity staining by formazan color, reaction and confirmed by enzyme assay. Isolated strain was identified by phenotypic and biochemical characteristics. Specific activity of falvoenzyme was also measured.
Results: In total of 400 isolated bacteria, only one of them was PutA producer that recognized as Pseudomonas fluorescens. The specific activity of falvoenzyme for L-proline and
D1-Pyrroline-5-carboxylate was determined 22.4 U/mg and 10.7 U/mg, respectively.
Conclusion: Specific activity of this enzyme for proline and
D-pyrroline-5-carboxylate in compression with various amount of falvoenzyme which isolated from other resources is acceptable.

 
Keyword(s): ENZYME, PROLINE, PUTA FALVOENZYME, SCREENING, PSEUDOMONAS FLUORESENSE
 
 
References: 
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Click to Cite.
APA: Copy

SHAHBAZ MOHAMMADI, H., & OMIDINIA, E. (2009). ISOLATION, SCREENING AND IDENTIFICATION OF BI-FUNCTIONAL PUTA FLAVOENZYME PRODUCING PSEUDOMONAS FLUORESENSE FROM SOIL. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 1(1), 23-27. https://www.sid.ir/en/journal/ViewPaper.aspx?id=189768



Vancouver: Copy

SHAHBAZ MOHAMMADI H., OMIDINIA E.. ISOLATION, SCREENING AND IDENTIFICATION OF BI-FUNCTIONAL PUTA FLAVOENZYME PRODUCING PSEUDOMONAS FLUORESENSE FROM SOIL. JOURNAL OF MICROBIAL BIOTECHNOLOGY. 2009 [cited 2021May11];1(1):23-27. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=189768



IEEE: Copy

SHAHBAZ MOHAMMADI, H., OMIDINIA, E., 2009. ISOLATION, SCREENING AND IDENTIFICATION OF BI-FUNCTIONAL PUTA FLAVOENZYME PRODUCING PSEUDOMONAS FLUORESENSE FROM SOIL. JOURNAL OF MICROBIAL BIOTECHNOLOGY, [online] 1(1), pp.23-27. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=189768.



 
 
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