Paper Information

Journal:   JOURNAL OF IRANIAN ANATOMICAL SCIENCES   SUMMER 2010 , Volume 8 , Number 31; Page(s) 117 To 127.
 
Paper: 

DESIGNING OF HUMAN CARTILAGE TISSUE, BY DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS WITH BMP-6 IN ALGINATE SCAFFOLD

 
 
Author(s):  HASHEMI BANI B., RAZAVI SHAHNAZ*, ESFANDIARI EBRAHIM*, KARBASI S., MARDANI MOHAMMAD, NADALI FATEMEH, SADEGHI FARZANEH, HAGHIGHAT F., HAGHJOU SHAGHAYEGH, GOUHARIAN V.
 
* ANATOMICAL SCIENCES DEPARTMENT, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 
Purpose: In the present study the effect of BMP-6 was investigated on chondrogenesis of adiposederived stem cells.
Materials and Methods: Mesenchymal stem cells derived from subcutaneous adipose tissue were cultured on alginate scaffold to induce chondrogenesis in experimental group, with chondrogenic medium having BMP-6 growth factor for 3 weeks. In control group medium without BMP-6 was applied. The harvested constructs were examined with immunohistochemical and RT-PCR methods for assessment of cartilage-specific characteristics.
Results: The results of immunohistochemical method revealed the presence of typical cartilage extracellular matrix components such as type II collagen and aggrecan in constructs induced by BMP-6 growth factor on alginate scaffold. In addition evaluation of the results of RT-PCR analysis confirmed the expression of cartilage- specific genes, such as type II collagen and aggrecan, in the differentiated cells under the influence of growth factor BMP-6.
Conclusion: It can be concluded that BMP-6 promotes chondrogenesis of ADSC in 3-D and adipose-derived stem cells could be used for cartilage tissue engineering.
 
Keyword(s): ADIPOSE-DERIVED STEM CELLS, ALGINATE, BMP-6, TISSUE ENGINEERING, CHONDROGENESIS
 
References: 
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