Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   WINTER 2011 , Volume 12 , Number 4 (48); Page(s) 503 To 510.
 
Paper: 

PROTEOME ANALYSIS OF MOUSE BRAIN EXPOSED TO CHRONIC HYPOXIA

 
 
Author(s):  GILANI K.*, AERTS M., DEWILDE S., DEVREESE B., MOENS L., VAFAKHAH M.
 
* REPRODUCTIVE BIOTECHNOLOGY RESEARCH CENTER, AVICENNA RESEARCH INSTITUTE, SHAHID BEHESHTI UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain.
Materials and Methods: Using the emPAI protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7% O2 for 48 hours. Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression.
Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca2+ signaling, pentose phosphate pathway; 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.

 
Keyword(s): CHRONIC HYPOXIA, BRAIN, MOUSE, PROTEOMICS, GELC-MS/MS
 
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