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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   2010 , Volume 39 , Number 4; Page(s) 35 To 44.
 
Paper: 

PRELIMINARY IDENTIFICATION AND TYPING OF PATHOGENIC AND TOXIGENIC FUSARIUM SPECIES USING RESTRICTION DIGESTION OF ITS1-5.8S RDNA-ITS2 REGION

 
 
Author(s):  MIRHENDI H.*, GHIASIAN A., VISMER H.F., ASGARI M.R., JALALIZAND N., ARENDRUP M.C., MAKIMURA K.
 
* DEPT. OF MEDICAL PARASITOLOGY AND MYCOLOGY, SCHOOL OF PUBLIC HEALTH AND NATIONAL INSTITUTE OF HEALTH RESEARCH, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: Fusarium species are capable of causing a wide range of crop plants infections as well as uncommon human infections. Many species of the genus produce mycotoxins, which are responsible for acute or chronic diseases in animals and humans. Identification of Fusaria to the species level is necessary for biological, epidemiological, pathological, and toxicological purposes. In this study, we undertook a computer-based analysis of ITS1-5.8SrDNA-ITS2 in 192 GenBank sequences from 36 Fusarium species to achieve data for establishing a molecular method for specie-specific identification.
Methods: Sequence data and 610 restriction enzymes were analyzed for choosing RFLP profiles, and subsequently designed and validated a PCR-restriction enzyme system for identification and typing of species. DNA extracted from 32 reference strains of 16 species were amplified using ITS1 and ITS4 universal primers followed by sequencing and restriction enzyme digestion of PCR products.
Results: The following 3 restriction enzymes TasI, ItaI and CfoI provide the best discriminatory power. Using ITS1 and ITS4 primers a product of approximately 550bp was observed for all Fusarium strains, as expected regarding the sequence analyses. After RFLP of the PCR products, some species were definitely identified by the method and some strains had different patterns in same species.
Conclusion: Our profile has potential not only for identification of species, but also for genotyping of strains. On the other hand, some Fusarium species were 100% identical in their ITS-5.8SrDNA-ITS2 sequences, therefore differentiation of these species is impossible regarding this target alone. ITS-PCR-RFLP method might be useful for preliminary differentiation and typing of most common Fusarium species.

 
Keyword(s): FUSARIUM, MYCOTOXINS, PCR-RFLP, ITS
 
 
References: 
 
Click to Cite.
APA: Copy

MIRHENDI, H., & GHIASIAN, A., & VISMER, H., & ASGARI, M., & JALALIZAND, N., & ARENDRUP, M., & MAKIMURA, K. (2010). PRELIMINARY IDENTIFICATION AND TYPING OF PATHOGENIC AND TOXIGENIC FUSARIUM SPECIES USING RESTRICTION DIGESTION OF ITS1-5.8S RDNA-ITS2 REGION. IRANIAN JOURNAL OF PUBLIC HEALTH, 39(4), 35-44. https://www.sid.ir/en/journal/ViewPaper.aspx?id=189107



Vancouver: Copy

MIRHENDI H., GHIASIAN A., VISMER H.F., ASGARI M.R., JALALIZAND N., ARENDRUP M.C., MAKIMURA K.. PRELIMINARY IDENTIFICATION AND TYPING OF PATHOGENIC AND TOXIGENIC FUSARIUM SPECIES USING RESTRICTION DIGESTION OF ITS1-5.8S RDNA-ITS2 REGION. IRANIAN JOURNAL OF PUBLIC HEALTH. 2010 [cited 2021May11];39(4):35-44. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=189107



IEEE: Copy

MIRHENDI, H., GHIASIAN, A., VISMER, H., ASGARI, M., JALALIZAND, N., ARENDRUP, M., MAKIMURA, K., 2010. PRELIMINARY IDENTIFICATION AND TYPING OF PATHOGENIC AND TOXIGENIC FUSARIUM SPECIES USING RESTRICTION DIGESTION OF ITS1-5.8S RDNA-ITS2 REGION. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 39(4), pp.35-44. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=189107.



 
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