Paper Information

Journal:   HEPATITIS MONTHLY   JANUARY 2011 , Volume 11 , Number 1 (30); Page(s) 14 To 18.
 
Paper: 

A STUDY OF GENOTYPES, MUTANTS AND NUCLEOTIDE SEQUENCE OF HEPATITIS B VIRUS IN PAKISTAN

 
 
Author(s):  MUMTAZ KH.*, HAMID S., AHMED SH., MOATTER T., MUSHTAQ SH., KHAN A., MIZOKAMI M., JAFRI W.
 
* DEPARTMENTS OF MEDICINE AND PATHOLOGY, AGA KHAN UNIVERSITY, KARACHI, PAKISTAN
 
Abstract: 

Background: Hepatitis B virus (HBV) genotypes and mutations are gaining importance in determining the clinical course of chronic liver disease.
Objectives: To determine and compare the distribution of HBV genotypes and genomic variations in Pakistan to other parts of the world. Patients and Methods: We conducted a prospective study at Aga Khan University Hospital from December 2006 to December 2008. HBV genotype was determined in 257 HBV DNA-positive patients. Patients were divided into two groups according to HBeAg positivity. Mutations in the pre-core and core promoter regions of HBV were determined in HBeAg-negative patients by line probe INNOLIPA assay.
Results: The mean
±SD age of patients was 28±5 years; there were 201 (78%) men. HBeAg was positive in 219 (85%) patients and negative in 38 (15%). HBeAg-positive patients were younger than HBeAgnegative patients (95% vs 21% in£30 years, p<0.001). HBV genotype D found in 247 (96.2 %) patients followed by a combined infection with HBV genotype B+D in 9 (3.3%) and 1 (0.5%) with genotype A. The mutations identified in 38 HBeAg-negative patients were T1762/A1764 in 21 (55.2%), PC mutant in7 18.4, (%T1762/A1764/PC mutant in 2 (5%) and T1762/A1764/PC wild mutation in 1 (2%); no mutation identified in 7 (18.4%). Phylogenetic analysis did not show any significant differences between HBV genotype D isolated from Pakistan and those isolated from other parts of the world. Conclusions: HBV genotype D is predominant in Pakistan, irrespective of HBeAg status. PC and BCP mutations were found in significant numbers of patients infected with genotype D. The HBV genotype D isolates from Pakistan are identical to the sequences isolated from other parts of the world.

 
Keyword(s): HBV, GENOTYPE, HEPATITIS B, EPIDEMIOLOGY, MUTANTS, DNA
 
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