Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   WINTER 2011 , Volume 12 , Number SUPPLEMENT 1 (THE 1ST INTERNATIONAL STUDENT CONGRESS ON CELL AND MOLECULAR MEDICINE); Page(s) 106 To 107.
 
Paper: 

STEM CELL AND REGENERATIVE MEDICINE: P-148: PPARγ1 EXPRESSION PROFILE DURING DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO DEFINITIVE ENDODERM

 
 
Author(s):  SHABANI KH.*, GHAEDI K., NASR ESFAHANI M.H., KARBALAEI KH., FAROKHI A., TANHAEI S.
 
* ROYAN INSTITUTE, ISFAHAN, IRAN
 
Abstract: 
Objective: The PPARs (peroxisome proliferatoractivated receptors) are members of the nuclear hormone receptor superfamily. Three major isoforms of PPARs (a, b and g) have been identified that each with a possibility of different ligands, target genes and biological roles.
The expression of PPAR
a, b and g varies widely from tissue-to-tissue. PPAR g has been demonstrated to play an important role in the regulation of cell differentiation.
PPAR
g has 2 isoforms termed: g1, g2, which are arisen by differential transcription start sites and alternative splicing. In order to see the importance of PPAR g1 in differentiation process of embryonic stem cells, the present study was undertaken.
Materials and Methods: Endodermal differentiation of mESCs (Royan B1 cell lines) cells was induced by culturing the cells in N2B27 media including for 5 days under treatment by Activin A at final concentration of 100 ng/ml with daily medium change. Treatment pursued by the same condition supplemented with retinoic acid (RA) (0.1
mM) in place of Activin A for more three days. In order to chase the mRNA level of PPAR g1, Total RNA was extracted from mESC, cells before RA treatment (d5) and after reaching to the endodermal cell fate (d8, DE) and cDNA synthesis was carried out to perform a real time quantification analysis for PPAR g1.
Results: Despite a decrease in expression pattern of PPAR
g1 in d5 cells, the results revealed an increasing expression pattern of PPARg1 in DE differentiated cells (d8, DE) relative to mESC and d5, which was significant at p < 0.05.
Conclusion: In the present research, we have attempted to have a further look into the expression level of PPAR
g1 gene during the differentiation of stem cells to definitive endoderm. In conclusion, our data clearly indicated that an increment in PPAR g1 upon RA treatment may be somehow is involved in regulation of stem cells differentiation in to definitive endoderm which could be clarified in further studies.
 
Keyword(s): PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS, MOUSE EMBRYONIC STEM CELLS, DEFINITIVE ENDODERM
 
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