Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   WINTER 2011 , Volume 12 , Number SUPPLEMENT 1 (THE 1ST INTERNATIONAL STUDENT CONGRESS ON CELL AND MOLECULAR MEDICINE); Page(s) 97 To 97.
 
Paper: 

PHARMACOLOGY: P-129: ANTIGENOTOXIC EFFECT OF DISSULFIDE COMPOUNDS FROM "FERULA PERSICA WILLD" ON RAT LYMPHOCYTES EXPOSED TO OXIDATIVE STRESS

 
 
Author(s):  TAGHIABADI E.*, KARIMI G., BEHRAVAN J., IRANSHAHI M., MOSAFA F., NOROUZI S., RAZAVI M.
 
* DEPARTMENT OF TOXICOLOGY AND PHARMACOLOGY, SCHOOL OF PHARMACY, UNIVERCITY OF MEDICAL SCIENCE, MASHHAD, IRAN
 
Abstract: 

Objective: In the present study, the antigenotoxic effect of disulfide compounds from Ferula persica on DNA damage in healthy rat lymphocytes induced by H2O2 was evaluated using single-cell electrophoresis (Comet assay). Sulfur containing compounds previously reported from F. persica, were persica sulfide A (PSA) and persica sulfide B (PSB). In our study two sulfur containing compounds were extracted and characterized by NMR.
These two compounds were then identified as PSA and a new compound that after characterization was named persica sulfide C (PSC). Since the amount of extracted PSC was insufficient for comet assay, the antioxidant/ antigenotoxic effects of PSA were investigated alone.
Materials and Methods: Effect of solutions containing 1, 10, 50, 100, 200, 300, 400 and 500
mM of PSA, these concentration and 250, 500, 750, 1000 mM of ascorbic acid in the presence of hydrogen peroxide (H2O2) 25mM and 25, 50, 100 and 200 mM of H2O2 was tested on lymphocytes derived from the blood of healthy male Wistar rats (250-300 g), were measured by comet assay. The degree of damage to DNA after exposure to different solutions at 4° for 15 minutes was calculated based the amount of DNA present in the tail compared to the total amounts of lymphocyte DNA.
Results: The results indicated that PSA did not have genetoxicity. And also it made a 50% reduction in DNA damage caused by H2O2 (EC 50) at the concentration 476.47 (360.28-630.13) μM. According to the calculated EC 50 for ascorbic acid [1399.23 (1054.27-1857.07)], it can be deduced that PSA is more potent than ascorbic acid in reduction of oxidative DNA damages Conclusion: It is concluted that PSA did not have genetoxicityeffect and prevented the oxidative DNA damage to rat lymphocytes.

 
Keyword(s): FERULA PERSICA, PERSICA SULFIDE A, COMET ASSAY, ANTIOXIDANT
 
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