The aim of the present study was to investigate the prevalence of Campylobacter species in poultry livers in Isfahan. Between January 2006 to May 2008, a total of 205 poultry liver samples from chicken (n=110), turkey (n=65) and ostrich (n=30) liver were procured and analyzed from Isfahan city of Iran. In total, 101 Campylobacter were isolated from all of the liver samples analyzed, of which 85 (84.2%) were confirmed as Campylobacter jejuni and the remaining (15.8%) as Campylobacter coli. The highest prevalence of Campylobacter spp. was recorded in chicken liver (63.6%), followed by turkey liver (40%) and ostrich liver (16.7%). It was concluded that a high proportion of poultry liver marketed in Isfahan, was contaminated by Campylobacter spp. Hence, to ensure food safety, poultry livers must be cooked properly before consumption.