Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   WINTER 2011 , Volume 12 , Number SUPPLEMENT 1 (THE 1ST INTERNATIONAL STUDENT CONGRESS ON CELL AND MOLECULAR MEDICINE); Page(s) 78 To 78.
 
Paper: 

IMMUNOLOGY AND IMMUNOTHERAPY: P-85: MOLECULAR CLONING AND EXPRESSION OF RECOMBINANT HUMAN INTERLEUKIN-7

 
 
Author(s):  SADEGHI I.*, AHMADIAN N., BAHADORI Z., GHALANDARI M., BEHMANESH M.
 
* DEPARTMENT OF GENETICS, FACULTY OF BIOLOGICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Objective: Human interleukin- (IL-7) is a member of interleukin family that regulates the development and survival of lymphoid and myeloid cells. Recent clinical studies of IL-7 for treatment of various diseases, including immunodeficiency disorders and malignancies have shown promising results suggesting that IL-7 may facilitate additional therapeutic endeavors including bone marrow/organ transplantation and cancer immunotherapy, hence, it is considered to be produced in large amounts using prokaryotic and eukaryotic expression systems. Previously, a study reported that could be purified from inclusion bodies using a prokaryotic system, however, the required refolding step limits the recovery rates. Currently human IL-7 is produced from E. coli expression system which involves inclusion bodies that required refolding steps.
Materials and Methods: Using specific primers, the desired fragment of human IL-7 gene was amplified by PCR, its cDNA has been made and amplified and was cloned into the PET32a and then transformed into the BL21.
Results: The recombinant human IL-7 was successfully amplified, transformed, cloned and expressed in BL21.
Conclusion: The recombinant human IL-7 can be easily cloned and expressed in prokaryotic systems.

 
Keyword(s): IL-7, RECOMBINANT PROTEIN, MOLECULAR CLONING
 
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