Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   WINTER 2011 , Volume 12 , Number SUPPLEMENT 1 (THE 1ST INTERNATIONAL STUDENT CONGRESS ON CELL AND MOLECULAR MEDICINE); Page(s) 76 To 77.
 
Paper: 

IMMUNOLOGY AND IMMUNOTHERAPY: P-82: ASSESSMENT OF SOME CRITICAL CLINICOPATHOLOGICAL FACTORS AND KRAS POLYMORPHISM IN COLORECTAL CARCINOMA PATIENTS

 
 
Author(s):  LARI S.*, GHAFARZADEGAN K., AFSHARNEZHAD S., SARAF YAZDI M., PAZOUOKI N., HOSSEINKHANI S.
 
* DEPARTMENT OF BIOLOGY, SCIENCE AND RESEARCH BRANCH, MASHHAD ISLAMIC AZAD UNIVERSITY, MASHHAD, IRAN
 
Abstract: 

Objective: Colorectal cancer is one of the most common forms of cancer in the world. AmongSeveral genetic events that have been documented in colorectal tumour progression, K-ras has high diagnostic value .studies have shown that K-ras mutations are found in 20-50% 0f all colon cancer mostly in codons 12 and 13, and less frequently in codon 61.The aim of this study is to identify K-ras gene mutations in codon 13 in Iranian CRC patients and to assess whether they are linked with the clinicopathological parameters.
Materials and Methods: Tumor samples were collected from a consecutive series of 54 patients undergoing resective surgery for CRC. DNA was extracted from tumour. We applied RFLP-PCR for codon 13 mutation detection.
Results: Colorectal patients, 18.5%, presented with mutations in codon 13 of K-ras. In our study K-ras mutations were significantly associated with CRC stage and age (p>0.05). No significant correlations were found between the mutations and sex, grade and tumor location.
Conclusion: According to significant correlation between age and tumor stage with mutation in codon 13 of K-ras, we expect younger patients with colon cancer and lower response rate to treatment and higher stage in our country.

 
Keyword(s): COLORECTAL CANCER, STAGE, K-RAS, RFLPPCR
 
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