Paper Information

Journal:   DARU JOURNAL OF PHARMACEUTICAL SCIENCE   2003 , Volume 11 , Number 1; Page(s) 34 To 37.
 
Paper: 

HYOSCYAMINE 6 B-HYDROXYLASE GENE ISOLATION FROM IN VITRO CULTURED ROOTS OF HYOSCYAMUS NIGER L. AND HYOSCAYMUS TENUICAULIS SCHONBECK-TEMESY

 
 
Author(s):  EBRAHIMZADEH H.*, TEYMOURI A., LOHRASEBI T.
 
* DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCE, UNIVERSITY OF TEHRAN
 
Abstract: 

The H6H gene for hyoscyamine 6β-hydroxylase, which converts hyoscyamine to scopolamine, was isolated from H. niger and H. tenuicaulis. The roots of 14 days sterile seedlings were transferred to a modified liquid B-5 medium containing 1m M indolebutyric acid, and after appearance of the lateral roots, sub cultured in a free hormone medium. Following a week, the total cellular RNA of the roots was extracted. cDNA of H6H gene was synthesized by RT-PCR, favored gene was amplified by the PCR.

 
Keyword(s): HYOSCYAMUS NIGER, HYOSCYAMUS TENUICAULIS, CULTURED ROOTS, H6H GENE, CDNA SYNTHESIS
 
References: 
 
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