Paper Information

Journal:   IRANIAN JOURNAL OF PEDIATRICS   Spring 2003 , Volume 13 , Number 1; Page(s) 3 To 10.
 
Paper: 

LIVE TRANSPLANTATION IN CHILDREN ANTIBODY PRODUCTION AGAINST ِE. COLI POLYPEPTIDE (ECP) BY DOT BLOTTING AND ELISA METHODS FOR ECP ASSAY IN RECOMBINANT DRUG AND PATIENTS' SERA

 
 
Author(s):  AHANGARI GHASEM, AKRAMI H., SANATI M.H., RABANI ALI
 
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Abstract: 

Recombinant DNA technology made it possible to produce biological drugs and pharmaceutical companies produced recombinant human drugs. There have been reports on the formation of antibodies against residual host cell protein. For this reason, it is important to check the patients sera for antibodies which could possibly be induced by recombinant drug it self and/or by contaminating proteins of the host cell. The present report refers to the obtainment of polyspecific antisera directed against Escherichia coli host strain used to produce recombinant human growth hormone. The antisera were obtained by the cascade immunization method. The animals (n=3 rabbits) were initially immunized with E. coli strain. The analysis of the antisera by dot blotting method showed a progressive recognition of the cascade method. These antisera were also used by ELISA methods. Results showed antibodies rising against E. coli polypeptide antigen.

 
Keyword(s): POLYPEPTIDES, E. COLI, RECOMBINANT DRUGS, IMMUNOGENECITY, DOT BLOTTING
 
References: 
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