Paper Information

Journal:   DARU JOURNAL OF PHARMACEUTICAL SCIENCE   2005 , Volume 13 , Number 1; Page(s) 11 To 16.
 
Paper: 

SIMULTANEOUS DETERMINATION OF ANTHOCYANOSIDE AND BETA-CAROTENE BY THIRD-DERIVATIVE ULTRAVIOLET SPECTROPHOTOMETRY

 
 
Author(s):  SOURI E., JALALIZADEH H., FARSAM H., REZWANI H., AMANLOU M.
 
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Abstract: 
Derivative spectrophotometry offers a useful approach for the analysis of drugs in multi-component mixtures. In this study a third-derivative spectrophotometry method was used for simultaneous determination of anthocyanoside and beta-carotene using the zero-crossing technique. The measurements were carried out at wavelengths of 625 and 540 nm for anthocyanoside and beta-carotene respectively.
The method was found to be linear (r2>0.999) in the range of 125-750 µg/mL for anthocyanoside in the presence of 25 µg/mL beta-carotene at 625 nm. The same linear correlation was also obtained (r2>0.997) in the range of 6.25-37.50 µg/mL for beta-carotene in the presence of 500 µg/mL of anthocyanoside at 540 nm. The limit of determination was 125 and 6.25 µg/mL for anthocyanoside and beta-carotene respectively. The method was successfully applied for simultaneous determination of anthocyanoside and beta-carotene in pharmaceutical preparations without any interferences from excipients.
 
Keyword(s): ANTHOCYANOSIDE , BETA-CAROTENE , THIRD DERIVATIVE SPECTROPHOTOMETRY , SIMULTANEOUS
 
References: 
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