Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   December 2001 , Volume 37 , Number 3-4; Page(s) 247 To 272.
 
Paper: 

SOURCES AND PROPERTIES OF IRANIAN ISOLATE OF PEA ENATION MOSAIC VIRUS

 
 
Author(s):  FARZADFAR S., IZADPANAH K.A.
 
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Abstract: 

Mechanical inoculation of the Iranian isolate of Pea enation mosaic virus (PEMV-I) induced local lesions in Chenopodium amaranticolor and C. quiona and systemic vein clearing and mosaic in Lathyrus odoratus, Lens culinaris, Medicago sativa, Melilotus officinalis, Phaseolus vulgaris cv. Bountiful, Pisum sativum, Trifolium alexandrinum, T. repens and Vicia faba. Infected Glycyrrhiza glabra showed no symptoms. Amaranthus retroflexus,Chenopodium album, Glycine max, Phaseolus vulgaris cv. Goli, Top crop, and Kentucky, Pheseolus aureus cv. Gohar and Vigna unguiculata were not susceptible.
PEMV-I was purified by methods of lzadpanah and Shepherd (1966) and Mahmood and Peters (1973). In density-gradient centrifugation two bands were distinguished, a weak top (T) and a strong bottom (B) band. Both components contained small polyhedral particles of 28-30 nm diameter as revealed byelecteron microscopy. Virus yield was 35 mg and 12.6 mg per 100g of infected tissue by the methods of Izadpanah and Shepher (1966) and Mahmood and Peters (1973), respectively. Use of Triton X-100 increased the yield.
DAS-ELISA with PEMV-I-IgG showed that Trifolium alexandrinum, T. repens, Medicago sativa, Melilotus officinalis and Glycyrrhiza glabra are natural sources of PEMV-I in Bajgah and Trifolium alexandrinumn is a natural host of the virus in Karaj. This is the first report of G. glabra and T. alexandrinum as the hosts of PEMV.
In SDS-PAGE and western blot analysis, the molecular weight of the virus structral proteins were estimated at about 56000 and 22000 daItons. A 44000 daiton protein band detected in SDS-PAGE and western blot was probably a dimer of 22000 daiton protein. In agarose gel electerophoresis, values of 1.7x106 and l.4x106 daltons were obtained for RNA-1 and RNA-2, respectively. No satellite RNA was detected.
On the basis of biological and serological properties, the Iranian isolate of PEMV is quite similar to the isolates reported elsewhere. Also, it was found that Pisum salivum, Cicer arietinum, Vicia saliva, ViCia faba and Glycine max are natural hosts of PEMV in Fars, Ghazvin, Golestan, Lorestan, Mazandaran and Tehran provinces of Iran.

 
Keyword(s): PEA ENATION MOSAIC VIRUS, LEGUME VIRUSES, PEA, LICORICE, ALFALFA, CLOVER, SOYBEAN, VIRUS PURIFICATION, VIRUS SOURCES
 
References: 
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